{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T03:54:14Z","timestamp":1791431654619,"version":"4.3.3"},"reference-count":46,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Extracellular Vesicle"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Lethal prostate cancer (PCa) is dominated by treatment\u2010refractory progression in bone. In this study, we used plasma\u2010derived extracellular vesicles (EVs) for monitoring the tumour and therapy\u2010induced changes in the bone tumour microenvironment (bone\u2010TME) in men with bone metastatic PCa (M1b PCa). Transcriptome analysis of differentially expressed genes in plasma EVs from patients with M1b and localized (M0) PCa, identified a \u2018bone metastasis signature\u2019 (BMS) that reflects tumour\u2010induced bone formation. In preclinical studies, the BMS was enriched in plasma EVs from mice inoculated with bone\u2010metastatic MDA PCa\u2010118b but not with adrenal\u2010metastatic MDA PCa\u2010250 tumours. Longitudinal monitoring of the BMS for up to 50 days showed that the levels of several genes increased prior to detectable growth of bone\u2010forming tumours. Real\u2010time monitoring of the BMS from tumour\u2010bearing mice treated with the bone\u2010targeting agent Ra\u2010223 showed reduction of their expression that correlated with reduction in bone formation and tumour growth. Development of resistance to Ra\u2010223 correlated with the induction of the BMS genes. In a cohort of PCa patients with clinically localized PCa with pathologic lymph node involvement, increases in BMS genes following hormonal therapy correlated with the subsequent development of metastasis during long term follow up. The BMS may enable longitudinal monitoring of the initiation, progression and treatment response of M1b PCa.<\/jats:p>","DOI":"10.1002\/jev2.70364","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T03:31:47Z","timestamp":1791430307000},"source":"Crossref","is-referenced-by-count":0,"title":["Longitudinally Monitoring Prostate Cancer Bone Metastasis and Treatment Response via Circulating Extracellular Vesicles"],"prefix":"10.1002","volume":"15","author":[{"given":"Emanuela","family":"Gentile","sequence":"first","affiliation":[{"name":"Department of GU Medical Oncology The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul G.","family":"Corn","sequence":"additional","affiliation":[{"name":"Department of GU Medical Oncology The University of Texas MD Anderson Cancer Center  Houston Texas 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