{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:01:40Z","timestamp":1790697700522,"version":"4.1.0"},"reference-count":97,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Extracellular Vesicle"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Small\u00a0extracellular vesicles (sEVs) carry diverse cargo essential for intercellular communication, but their organism\u2010wide characterization across all tissues is still limited. Here, we present a multi\u2010omics sEV atlas covering the whole\u2010body of 120 tissue sites, four body fluids and nine gastrointestinal contents from the same pig. We identified 7995 proteins, 337 miRNAs as well as 105,409 transcripts (93,409 full\u2010length) in sEVs by mass spectrometry, next\u2010 and third\u2010generation RNA sequencing. Among these, a conserved cargo set of 252 proteins, 18 miRNAs and 1227 transcripts was defined, including RNA\u2010binding proteins (ANXA2, ALIX and MVP), mitochondrial proteins (ATP5F1A, COX6C and VDACs), the let\u20107 miRNA family and ferritin genes (\n                    <jats:italic>FTH1<\/jats:italic>\n                    \/\n                    <jats:italic>FTL<\/jats:italic>\n                    ). Notably, sEV biogenesis\u2010associated ACTG1, CFL1 and ANXA2 were detected in all samples, suggesting their potential as novel sEV protein markers. In contrast, 2638 proteins, 152 miRNAs and 1000 transcripts showed organ\u2010specific expression patterns, which are likely to underpin organ\u2010specific biological processes. Moreover, by using this multi\u2010omic dataset and additional RIP assays, we found that HNRNPU participates in the sorting of hypoxia\u2010responsive\n                    <jats:italic>EPAS1<\/jats:italic>\n                    into sEVs. This comprehensive sEV atlas establishes a foundation for future studies in sEV cell biology.\n                  <\/jats:p>","DOI":"10.1002\/jev2.70374","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:03:38Z","timestamp":1790694218000},"source":"Crossref","is-referenced-by-count":0,"title":["Conserved and Tissue\u2010Specific RNA and Protein Cargos of Small Extracellular Vesicles From 120 Tissue Sites Across 34 Organs in a Single Pig"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-8767-1991","authenticated-orcid":false,"given":"Naixiang","family":"Yu","sequence":"first","affiliation":[{"name":"National Key Laboratory for Pig Genetic Improvement and Germplasm Innovation Jiangxi Agricultural University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinxiu","family":"Liu","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Pig Genetic Improvement and Germplasm Innovation Jiangxi Agricultural University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-4475-7187","authenticated-orcid":false,"given":"Chenliang","family":"Lai","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Pig Genetic Improvement and Germplasm Innovation Jiangxi Agricultural University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyi","family":"Liu","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Pig Genetic Improvement and Germplasm Innovation Jiangxi Agricultural University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1811-9210","authenticated-orcid":false,"given":"Jiacheng","family":"Wei","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Pig Genetic Improvement and Germplasm Innovation Jiangxi Agricultural University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0853-7828","authenticated-orcid":false,"given":"Jiahong","family":"Zhong","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Pig Genetic Improvement and Germplasm Innovation Jiangxi Agricultural University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanya","family":"Xiong","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Pig Genetic Improvement and Germplasm Innovation Jiangxi Agricultural University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingjie","family":"Zeng","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Pig Genetic Improvement and Germplasm Innovation Jiangxi Agricultural University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junwu","family":"Ma","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Pig Genetic Improvement and Germplasm Innovation Jiangxi Agricultural University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhou","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Pig Genetic Improvement and Germplasm Innovation Jiangxi Agricultural University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2387-8735","authenticated-orcid":false,"given":"Zhiyan","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Pig Genetic Improvement and Germplasm Innovation Jiangxi Agricultural University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"crossref","DOI":"10.1002\/jex2.106","article-title":"Survey of Organ\u2010Derived Small Extracellular Vesicles and Particles (sEVPs) to Identify Selective Protein Markers in Mouse Serum","volume":"2","author":"Abdelmohsen K.","year":"2023","journal-title":"Journal of Extracellular Biology"},{"key":"e_1_2_11_3_1","doi-asserted-by":"crossref","first-page":"W72","DOI":"10.1093\/nar\/gkab393","article-title":"Cat RAPID Omics v2.0: Going Deeper and Wider in the Prediction of Protein\u2013RNA Interactions","volume":"49","author":"Armaos A.","year":"2021","journal-title":"Nucleic Acids Research"},{"key":"e_1_2_11_4_1","doi-asserted-by":"crossref","first-page":"3025","DOI":"10.1172\/JCI68782","article-title":"Type 2 Alveolar Cells Are Stem Cells in Adult Lung","volume":"123","author":"Barkauskas C. E.","year":"2013","journal-title":"Journal of Clinical Investigation"},{"key":"e_1_2_11_5_1","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.adi4386","article-title":"miRNA Cargo in Circulating Vesicles From Neurons is Altered in Individuals With Schizophrenia and Associated With Severe Disease","volume":"9","author":"Barnett M. M.","year":"2023","journal-title":"Science Advances"},{"key":"e_1_2_11_6_1","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/nature18282","article-title":"Stem Cell Function and Stress Response are Controlled by Protein Synthesis","volume":"534","author":"Blanco S.","year":"2016","journal-title":"Nature"},{"key":"e_1_2_11_7_1","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1161\/CIRCULATIONAHA.122.063402","article-title":"Multiomics of Tissue Extracellular Vesicles Identifies Unique Modulators of Atherosclerosis and Calcific Aortic Valve Stenosis","volume":"148","author":"Blaser M. C.","year":"2023","journal-title":"Circulation"},{"key":"e_1_2_11_8_1","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A Flexible Trimmer for Illumina Sequence Data","volume":"30","author":"Bolger A. 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P.","year":"2016","journal-title":"Nucleic Acids Research"},{"key":"e_1_2_11_13_1","doi-asserted-by":"crossref","DOI":"10.1002\/imt2.107","article-title":"Ultrafast One\u2010Pass FASTQ Data Preprocessing, Quality Control, and Deduplication Using Fastp","volume":"2","author":"Chen S.","year":"2023","journal-title":"iMeta"},{"key":"e_1_2_11_14_1","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1038\/s41573-022-00410-w","article-title":"Therapeutically Harnessing Extracellular Vesicles","volume":"21","author":"Cheng L.","year":"2022","journal-title":"Nature Reviews Drug Discovery"},{"key":"e_1_2_11_15_1","doi-asserted-by":"crossref","first-page":"D1694","DOI":"10.1093\/nar\/gkad1007","article-title":"Vesiclepedia 2024: An Extracellular Vesicles and Extracellular Particles Repository","volume":"52","author":"Chitti S. 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L.","year":"2025","journal-title":"Nature Neuroscience"},{"key":"e_1_2_11_30_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2021.110277","article-title":"Tissue Differences in the Exosomal\/Small Extracellular Vesicle Proteome and Their Potential as Indicators of Altered Tissue Metabolism","volume":"38","author":"Garcia\u2010Martin R.","year":"2022","journal-title":"Cell Reports"},{"key":"e_1_2_11_31_1","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1038\/s41586-021-04234-3","article-title":"MicroRNA Sequence Codes for Small Extracellular Vesicle Release and Cellular Retention","volume":"601","author":"Garcia\u2010Martin R.","year":"2022","journal-title":"Nature"},{"key":"e_1_2_11_32_1","doi-asserted-by":"crossref","DOI":"10.1002\/btm2.10609","article-title":"Extracellular Vesicles From Normal Tissues Orchestrate the Homeostasis of Macrophages and Attenuate Inflammatory Injury of Sepsis","volume":"9","author":"Ge X.","year":"2024","journal-title":"Bioengineering & Translational Medicine"},{"key":"e_1_2_11_33_1","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1038\/s41586-022-05035-y","article-title":"Transcriptome Variation in Human Tissues Revealed by Long\u2010Read Sequencing","volume":"608","author":"Glinos D. 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