{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T17:48:59Z","timestamp":1791654539174,"version":"4.3.4"},"reference-count":79,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:00:00Z","timestamp":1791590400000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100011852","name":"Paracelsus Medizinische Privatuniversit\u00e4t","doi-asserted-by":"publisher","award":["2023\u2010IiF\u2010015\u2010Russe(MIGRAINE,InvestinFuture)"],"award-info":[{"award-number":["2023\u2010IiF\u2010015\u2010Russe(MIGRAINE,InvestinFuture)"]}],"id":[{"id":"10.13039\/501100011852","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011852","name":"Paracelsus Medizinische Privatuniversit\u00e4t","doi-asserted-by":"publisher","award":["ID469(FIZTalentPool)"],"award-info":[{"award-number":["ID469(FIZTalentPool)"]}],"id":[{"id":"10.13039\/501100011852","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011852","name":"Paracelsus Medizinische Privatuniversit\u00e4t","doi-asserted-by":"publisher","award":["2025\u2010IiF\u2010023\u2010Benedetti(REGAIN,InvestinFuture))"],"award-info":[{"award-number":["2025\u2010IiF\u2010023\u2010Benedetti(REGAIN,InvestinFuture))"]}],"id":[{"id":"10.13039\/501100011852","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Extracellular Vesicle"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Extracellular vesicles (EVs) mediate cell\u2010to\u2010cell communication in development, health and disease. Heparin is a common component in human induced pluripotent stem cell (hiPSC) media but its impact on hiPSC\u2010EVs beyond stabilizing growth factors is unknown. Published evidence indicates heparin dose\u2010dependent EV uptake inhibition in heterotypic target cells. We asked if heparin affects hiPSC\u2010EV yield, storage, phenotype, or function. We used large\u2010scale hiPSC\u2010EV propagation to obtain pro\u2010angiogenic hiPSC\u2010EVs in presence or absence of escalating heparin doses and determined EV quantity, identity and functionality. Heparin delayed hiPSC colony\u2010confluence without significantly affecting hiPSC viability, pluripotency markers and proliferation. Significantly elevated tetraspanin\n                    <jats:sup>+<\/jats:sup>\n                    \/flotillin\n                    <jats:sup>+<\/jats:sup>\n                    hiPSC\u2010EV number (mean 367\u00b131\u00a0EVs\/cell\/24\u00a0h at 200\u00a0IU heparin\/mL) enabled efficient purification of &gt;\u00a010\n                    <jats:sup>11<\/jats:sup>\n                    \/mL functional EVs at superior particle\/protein ratio &gt;\u00a010\n                    <jats:sup>10<\/jats:sup>\n                    \/mg. Heparin dose\u2010dependently inhibited EV uptake in hiPSC and endothelial progenitors, comparable to 5\u00a0IU\/mL flavobacterium heparinase, and increased EV recovery after storage in NaCl but not in cell\u2010conditioned media. EVs obtained from hiPSC at 1% O\n                    <jats:sub>2<\/jats:sub>\n                    with 200\u00a0IU\/mL heparin maintained superior angiogenesis similarly to control hiPSC\u2010EVs obtained without heparin. We conclude that heparin inhibits hiPSC\u2010EV reuptake and stabilizes NaCl\u2010cryopreserved EVs thus boosting EV recovery while maintaining pro\u2010angiogenic function. Heparin presence and dose should therefore be documented particularly in PSC media.\n                  <\/jats:p>","DOI":"10.1002\/jev2.70382","type":"journal-article","created":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T17:11:32Z","timestamp":1791652292000},"source":"Crossref","is-referenced-by-count":0,"title":["Heparin Can Augment Extracellular Vesicle Yield by Inhibiting Reuptake in Donor Cells During Cell Propagation"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1790-081X","authenticated-orcid":false,"given":"Andr\u00e9 Cronemberger","family":"Andrade","sequence":"first","affiliation":[{"name":"Cell Therapy Institute Paracelsus Medical University (PMU)  Salzburg Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5195-5155","authenticated-orcid":false,"given":"Rodolphe","family":"Poupardin","sequence":"additional","affiliation":[{"name":"Cell Therapy Institute Paracelsus Medical University (PMU)  Salzburg Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4795-8086","authenticated-orcid":false,"given":"Martin","family":"Wolf","sequence":"additional","affiliation":[{"name":"Cell Therapy Institute Paracelsus Medical University (PMU)  Salzburg Austria"},{"name":"Austrian Red Cross Research, OeRK  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0825-6839","authenticated-orcid":false,"given":"Liliia","family":"Paniushkina","sequence":"additional","affiliation":[{"name":"Cell Therapy Institute Paracelsus Medical University (PMU)  Salzburg Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6927-0854","authenticated-orcid":false,"given":"Karim","family":"Selmi","sequence":"additional","affiliation":[{"name":"Austrian Red Cross Research, OeRK  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8015-0735","authenticated-orcid":false,"given":"Deepika Kundully","family":"Suresh","sequence":"additional","affiliation":[{"name":"Austrian Red Cross Research, OeRK  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1324-5263","authenticated-orcid":false,"given":"Nicole","family":"Maeding","sequence":"additional","affiliation":[{"name":"Cell Therapy Institute Paracelsus Medical University (PMU)  Salzburg Austria"},{"name":"Austrian Red Cross Research, OeRK  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heide\u2010Marie","family":"Binder","sequence":"additional","affiliation":[{"name":"Cell Therapy Institute Paracelsus Medical University (PMU)  Salzburg Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8372-6225","authenticated-orcid":false,"given":"Janine","family":"Zwicklhuber","sequence":"additional","affiliation":[{"name":"Cell Therapy Institute Paracelsus Medical University (PMU)  Salzburg Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6766-4093","authenticated-orcid":false,"given":"Sarah","family":"Hochmann","sequence":"additional","affiliation":[{"name":"Cell Therapy Institute Paracelsus Medical University (PMU)  Salzburg Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9198-0857","authenticated-orcid":false,"given":"Andr\u00e9","family":"G\u00f6rgens","sequence":"additional","affiliation":[{"name":"Division of Biomolecular and Cellular Medicine Department of Laboratory Medicine Karolinska Institutet  Stockholm Sweden"},{"name":"Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST) Karolinska University Hospital  Huddinge Sweden"},{"name":"Karolinska ATMP Center ANA Futura Karolinska Institutet  Huddinge Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4468-9113","authenticated-orcid":false,"given":"Samir El","family":"Andaloussi","sequence":"additional","affiliation":[{"name":"Division of Biomolecular and Cellular Medicine Department of Laboratory Medicine Karolinska Institutet  Stockholm Sweden"},{"name":"Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST) Karolinska University Hospital  Huddinge Sweden"},{"name":"Karolinska ATMP Center ANA Futura Karolinska Institutet  Huddinge Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7204-0058","authenticated-orcid":false,"given":"Katharina","family":"Schallmoser","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine PMU  Salzburg Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1810-867X","authenticated-orcid":false,"given":"Dirk","family":"Strunk","sequence":"additional","affiliation":[{"name":"Cell Therapy Institute Paracelsus Medical University (PMU)  Salzburg Austria"},{"name":"Austrian Red Cross Research, OeRK  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"3890","DOI":"10.3390\/ijms22083890","article-title":"Hypoxic Conditions Promote the Angiogenic Potential of human Induced Pluripotent Stem Cell\u2010Derived Extracellular Vesicles","volume":"22","author":"Andrade A. 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