{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T03:24:01Z","timestamp":1788319441839,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,6,21]],"date-time":"2024-06-21T00:00:00Z","timestamp":1718928000000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21H03075"],"award-info":[{"award-number":["21H03075"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["isevjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Extracellular Bio"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Congenital diaphragmatic hernia (CDH) is a life\u2010threatening condition with high morbidity and mortality rates. The survival rate of neonates with severe CDH is reportedly only 10%\u201315%. However, prenatal prediction of severe cases is difficult, and the discovery of new predictive markers is an urgent issue. In this study, we focused on microRNAs (miRNAs) in amniotic fluid\u2010derived small EVs (AF\u2010sEVs). We identified four miRNAs (hsa\u2010miR\u2010127\u20103p, hsa\u2010miR\u2010363\u20103p, hsa\u2010miR\u2010493\u20105p, and hsa\u2010miR\u2010615\u20103p) with AUC\u00a0&gt;\u00a00.8 to classify good prognosis group and poor prognosis group in human study. The AUC for hsa\u2010miR\u2010127\u20103p and hsa\u2010miR\u2010615\u20103p, for predicting the poor prognosis, were 0.93 and 0.91, respectively. In addition, in the in vivo study, the miRNA profiles of the lung tissues of CDH rats were different from those of control rats. Additionally, two elevated miRNAs (rno\u2010miR\u2010215\u20105p and rno\u2010miR\u2010148a\u20103p) in the lung tissues of CDH rats were increased in the AF\u2010sEVs of CDH rats. Our results suggest that severe CDH neonates can be predicted prenatally with high accuracy using miRNAs contained in AF\u2010sEVs. Furthermore, miRNA profile changes in AF\u2010sEVs reflected the lung status in CDH. Our findings may contribute to the development of advanced perinatal care for patients with CDH.<\/jats:p>","DOI":"10.1002\/jex2.160","type":"journal-article","created":{"date-parts":[[2024,6,22]],"date-time":"2024-06-22T01:43:55Z","timestamp":1719020635000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Amniotic fluid\u2010derived small extracellular vesicles for predicting postnatal severe outcome of congenital diaphragmatic hernia"],"prefix":"10.1002","volume":"3","author":[{"given":"Seiko","family":"Matsuo","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Nagoya University Graduate School of Medicine  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0789-5102","authenticated-orcid":false,"given":"Akira","family":"Yokoi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Nagoya 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