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Our team explored the potential of serum\u2010derived large extracellular vesicles, so called microparticles\/microvesicles\/ectosomes, to serve as a supportive tool in decision\u2010making in polytrauma situations. We focused on whether monocyte derived large EVs may differentiate between polytrauma patients with internal organ injury (ISS\u00a0&gt;\u00a015) and those without. Thus, we compared our EV data to soluble biomarkers such as tumour necrosis factor alpha (TNF alpha) and Interleukin\u20108 (IL\u20108). From the blood of 25 healthy and 26 patients with polytrauma large EVs were isolated, purified, and characterized. TNF alpha and IL\u20108 levels were quantified. We found that levels of these monocyte derived large EVs were significantly higher in polytrauma patients with internal organ damage and correlated with the ISS. Interestingly, we also observed a decline in AnnV<jats:sup>+<\/jats:sup>CD14<jats:sup>+<\/jats:sup> large EVs during normal recovery after trauma. Thus, inflammatory serological markers as TNF alpha and as IL\u20108 demonstrated an inability to discriminate between polytrauma patients with or without internal organ damage, such as spleen, kidney, or liver lacerations\/ruptures. However, TNF and IL\u20108 levels were elevated in polytrauma cases overall when contrasted with healthy non\u2010traumatic controls. These findings suggest that delving deeper into the potential of AnnV<jats:sup>+<\/jats:sup> large EVs derived from monocytes could highly beneficial in the managment of polytrauma, potentially surpassing the efficacy of commonly used serum markers.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jex2.70005","type":"journal-article","created":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T05:31:03Z","timestamp":1725255063000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Monocyte derived large extracellular vesicles in polytrauma"],"prefix":"10.1002","volume":"3","author":[{"given":"Aliona","family":"W\u00f6hler","sequence":"first","affiliation":[{"name":"Department of General, Visceral and Thoracic Surgery German Armed Forces Central Hospital  Koblenz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine K.","family":"Gries","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I University Hospital Bonn of the Rheinische Friedrich\u2010Wilhelms\u2010University  Bonn Germany"},{"name":"Department of Internal Medicine II Saarland University Medical Center, Saarland University  Homburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebekka J. 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