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Brain\u2010derived extracellular vesicles (EVs) have been demonstrated to be implicated in AD pathogenesis by facilitating the propagation of Tau, amyloid\u2010\u03b2 and inflammatory cytokines. However, the impact of peripheral EVs (pEVs) in AD pathogenesis remains poorly investigated. The objective of our study was to compare the passage of pEVs from adults, cognitively healthy elderly, and AD patients through the blood\u2010brain barrier (BBB), to evaluate their uptake in the brain and to assess their impact on the microglia activity using in vitro and in vivo models. To this end, pEVs were enriched, characterized, and fluorescently labelled. The passage of pEVs through the endothelial bEnd.3 cells was studied in a Transwell device with either neuronal or microglia cells seeded at the bottom of the well. Following the internalization of pEVs from AD patients, microglia adopted an amoeboid morphology and released a heightened level of pro\u2010inflammatory cytokine IL\u20106. To further assess their in vivo transport across the BBB, pEVs were injected into the blood circulation of 2\u2010days post\u2010fertilization Tg(\n                    <jats:italic>flk1:EGFP<\/jats:italic>\n                    ) zebrafish. The biodistribution of pEVs was monitored at 1 and 24\u00a0h post\u2010injection using confocal microscopy. We demonstrated that pEVs traverse the BBB by transcytosis and subsequently diffuse progressively into the brain. pEVs were then internalized by neuronal and radial glial cells as seen in\n                    <jats:italic>Tg(huc:EGFP)<\/jats:italic>\n                    and\n                    <jats:italic>Tg(gfap:EGFP)<\/jats:italic>\n                    zebrafish, respectively. Additional experiments were performed with the intrahippocampal injection of pEVs in the mouse, indicating their spreading throughout the brain and their uptake by neuronal and glial cells. These findings contribute to novel insights into the fate of pEVs following their passage through the BBB in vitro and in vivo, and demonstrate for the first time that pEVs from AD patients affect microglia activity. This suggests a potential mechanism through which peripheral tissue cues may contribute to AD pathogenesis.\n                  <\/jats:p>","DOI":"10.1002\/jex2.70027","type":"journal-article","created":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T06:36:13Z","timestamp":1737095773000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Brain penetration of peripheral extracellular vesicles from Alzheimer's patients and induction of microglia activation"],"prefix":"10.1002","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2041-5134","authenticated-orcid":false,"given":"Hermine","family":"Counil","sequence":"first","affiliation":[{"name":"INRS Centre Armand\u2010Frappier Sant\u00e9 Biotechnologie  Laval Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rummenigge Oliveira","family":"Silva","sequence":"additional","affiliation":[{"name":"INRS Centre Armand\u2010Frappier 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