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However, therapeutic utility requires establishing effective extraction and purification protocols for manufacturing. Here, we systemically optimized an enzyme\u2010based EV extraction method and demonstrated the generalizability with different parts from seventeen medicinal plants, fresh, frozen or dried. The enzymatic pulping process (henceforth denoted as Phyto\u2010EVpure) of fresh ginger,\n                    <jats:italic>Morus alba<\/jats:italic>\n                    leaves and\n                    <jats:italic>Isatis indigotica Fort<\/jats:italic>\n                    . roots improved EV yield and purity over grinding. Ginger EVs processed via Phyto\u2010EVpure preserved bioactivities and conferred hepatoprotection in a non\u2010alcoholic fatty liver disease model in vitro and in vivo. Phyto\u2010EVpure efficiently isolated EVs from leaf, rhizome and root stored fresh, frozen or dried. Phyto\u2010EVpure enabled us to demonstrate that EVs from phloem of previously intractable dried vine samples exhibited greater antitumour activities than xylem in vitro. Collectively, we provided a manufacturing\u2010friendly protocol for medicinal plant EV isolation and demonstrated the generalizability of Phyto\u2010EVpure for efficient isolation of EVs from a wide spectrum of traditional medicinal plants under different conditions.\n                  <\/jats:p>","DOI":"10.1002\/jex2.70090","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T05:49:55Z","timestamp":1759211395000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Generalizability of Enzyme\u2010Based Isolation Approach for Extracellular Vesicles From Traditional Medicinal Plants"],"prefix":"10.1002","volume":"4","author":[{"given":"Qian","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Experimental Hematology &amp; The Province and Ministry Co\u2010sponsored Collaborative Innovation Center for Medical Epigenetics &amp; Schools of Medical Technology 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