{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T14:29:01Z","timestamp":1782484141211,"version":"3.54.5"},"reference-count":86,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T00:00:00Z","timestamp":1772496000000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["isevjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Extracellular Bio"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Preeclampsia (PE), a hypertensive disorder in pregnancy, is linked to placental vascular remodelling, increasing risks of foetal growth restriction and long\u2010term offspring health problems. The role of fetoplacental endothelial cell\u2010derived extracellular vesicles (EVs) in PE remains underexplored. This study investigates whether EV composition in Early\u2010Onset PE (EO\u2010PE) is altered, potentially contributing to impaired foetal development. Small EVs (sEVs) were isolated from primary fetoplacental endothelial cells (fpECs) of term (T), preterm (PT) and EO\u2010PE pregnancies. sEVs were characterised using transmission electron microscopy, nanoparticle tracking analysis and Western blotting, confirming spherical morphology, size (&lt;200\u00a0nm) and expression of canonical EV and endothelial markers. Proteomic profiling via nano\u2010LC MS\/MS and gene set enrichment analysis revealed a cohesive proteomic profile in fpEC\u2010derived T\u2010 and PT\u2010sEVs, but EO\u2010PE\u2010derived sEVs showed heterogeneity and functional alterations compared to T\u2010 and PT\u2010derived sEVs. Notably, EO\u2010PE\u2010sEVs were enriched in proteins affiliated to epithelial\u2010to\u2010mesenchymal transition and myogenesis, processes tied to tissue remodelling and vascular homeostasis, all hallmarks in PE. This signature may represent a molecular signal associated with endothelial dysfunction. In contrast, T\u2010sEVs were enriched in cell cycle and DNA repair pathways. These findings underscore the role of fetoplacental\u2010derived EVs in placental\u2010foetal communication under pathophysiological conditions.<\/jats:p>","DOI":"10.1002\/jex2.70122","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:19:57Z","timestamp":1772986797000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Proteomic Epithelial\u2010To\u2010Mesenchymal Transition Signature in Fetoplacental Small Extracellular Vesicles of Early\u2010Onset Preeclampsia"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-6528-6271","authenticated-orcid":false,"given":"Michaela","family":"Stoiber","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynaecology Medical University of Graz  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9440-9250","authenticated-orcid":false,"given":"Monika","family":"Horvat Mercnik","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Medical University of Graz  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2512-4750","authenticated-orcid":false,"given":"Birgit","family":"Hirschmugl","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Medical University of Graz  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6441-4072","authenticated-orcid":false,"given":"Barbara","family":"Darnhofer","sequence":"additional","affiliation":[{"name":"Core Facility Mass Spectrometry Medical University of Graz  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Pernitsch","sequence":"additional","affiliation":[{"name":"Core Facility Ultrastructure Analysis Medical University of Graz  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Leopold\u2010Posch","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Medical University of Graz  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8009-020X","authenticated-orcid":false,"given":"Ursula","family":"Hiden","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Medical University of Graz  Graz Austria"},{"name":"Research Unit Early Life Determinants Medical University of Graz  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6387-7446","authenticated-orcid":false,"given":"Dagmar","family":"Kolb","sequence":"additional","affiliation":[{"name":"Core Facility Ultrastructure Analysis Medical University of Graz  Graz Austria"},{"name":"Gottfried Schatz Research Center for Cell Signaling, Metabolism and Aging Division of Cell Biology, Histology and Embryology Medical University of Graz  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5589-8642","authenticated-orcid":false,"given":"Christian","family":"Wadsack","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Medical University of Graz  Graz Austria"},{"name":"BioTechMed\u2010Graz  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,3]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00794.2024"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2018.12.008"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1559\u20100275\u201011\u201040"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1055\/A\u20101938\u20100010"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751\u20107176.2010.00384.x"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0000000000003891"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178340"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.117.10803"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2023.0191"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577\u2010022\u201000763\u20108"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1515\/jpm.2010.126"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(20)32335\u20107"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u201012\u201035"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2022.03.039"},{"key":"e_1_2_12_16_1","unstructured":"R Core Team.2024.R: A Language and Environment for Statistical Computing.https:\/\/www.R\u2010project.org\/."},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201004468\u20100"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.14694"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/S00404\u2010025\u201008013\u20107"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1542\/PEDS.2011\u20103093"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14361"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572\u2010023\u201000417\u20106"},{"key":"e_1_2_12_23_1","unstructured":"Dinno A.2024.dunn.test: Dunn's Test of Multiple Comparisons Using Rank Sums.https:\/\/CRAN.R\u2010project.org\/package=dunn.test."},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/S41598\u2010023\u201045830\u20109"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2025.04.001"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.13383"},{"key":"e_1_2_12_27_1","volume-title":"An R Companion to Applied Regression (Third)","author":"Fox J.","year":"2019"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.earlhumdev.2011.04.006"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2008.083"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.9.5949"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2004.11.007"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964\u2010023\u201001286\u2010y"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2022.1061340"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/imt2.43"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw313"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2016.11.014"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/J.1365\u20102567.2012.03568.X"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.echo.2021.01.008"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/bimj.200810425"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596\u2010018\u20100082\u2010x"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.110.941203"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1159\/000430193"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1055\/S\u20100035\u20101565923"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1385762"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2025.01.001"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1159\/000100081"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432\u20100436.2008.00302.x"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.110.150235"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/pai.12344"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11233935"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra2109523"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamapsychiatry.2018.0854"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20082038"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1210\/endocr\/bqaa009"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.13521"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12311"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2018.02.006"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104939\u20107253\u20101_3"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.117.09321"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.70064"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.1213\/ANE.0000000000005812"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12884\u2010020\u201003084\u20107"},{"key":"e_1_2_12_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2016.08.078"},{"key":"e_1_2_12_64_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S208865"},{"key":"e_1_2_12_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preghy.2022.02.005"},{"key":"e_1_2_12_66_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.13860"},{"key":"e_1_2_12_67_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2017\u201000672"},{"key":"e_1_2_12_68_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0098667"},{"key":"e_1_2_12_69_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c230020"},{"key":"e_1_2_12_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2023.04.017"},{"key":"e_1_2_12_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2025.02.010"},{"key":"e_1_2_12_72_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0086791"},{"key":"e_1_2_12_73_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0056754"},{"key":"e_1_2_12_74_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471\u20100528.2010.02634.x"},{"key":"e_1_2_12_75_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.136"},{"key":"e_1_2_12_76_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2839"},{"key":"e_1_2_12_77_1","doi-asserted-by":"publisher","DOI":"10.5468\/OGS.2020.63.3.270"},{"key":"e_1_2_12_78_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10803\u2010007\u20100536\u20104"},{"key":"e_1_2_12_79_1","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2002.119638"},{"key":"e_1_2_12_80_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201039565\u20109"},{"key":"e_1_2_12_81_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12404"},{"key":"e_1_2_12_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2018.02.006"},{"key":"e_1_2_12_83_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-24277-4"},{"key":"e_1_2_12_84_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2023.04.006"},{"key":"e_1_2_12_85_1","doi-asserted-by":"publisher","DOI":"10.1186\/S12884\u2010025\u201007193\u2010Z"},{"key":"e_1_2_12_86_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2020.10.022"},{"key":"e_1_2_12_87_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201082733\u2010z"}],"container-title":["Journal of Extracellular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jex2.70122","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jex2.70122","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jex2.70122","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T08:41:10Z","timestamp":1774428070000},"score":1,"resource":{"primary":{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/10.1002\/jex2.70122"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":86,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1002\/jex2.70122"],"URL":"https:\/\/doi.org\/10.1002\/jex2.70122","archive":["Portico"],"relation":{},"ISSN":["2768-2811","2768-2811"],"issn-type":[{"value":"2768-2811","type":"print"},{"value":"2768-2811","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"2025-09-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70122"}}