{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T10:04:01Z","timestamp":1784887441536,"version":"3.55.0"},"reference-count":71,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22575024"],"award-info":[{"award-number":["22575024"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82302387"],"award-info":[{"award-number":["82302387"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["L222128"],"award-info":[{"award-number":["L222128"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7264406"],"award-info":[{"award-number":["7264406"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012236","name":"Beijing Institute of Technology Research Fund Program for Young Scholars","doi-asserted-by":"publisher","award":["XSQD\u2010202121010"],"award-info":[{"award-number":["XSQD\u2010202121010"]}],"id":[{"id":"10.13039\/501100012236","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["isevjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Extracellular Bio"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Temozolomide (TMZ) is a first\u2010line chemotherapeutic agent for the treatment of glioblastoma (GBM), while a majority of patients do not effectively respond to TMZ owing to the multiple resistance mechanisms. In this study, we found that TMZ treatment substantially increased the exosome secretion from GBM cell line. The secreted exosomes from TMZ\u2010treated C6 cells maintained typical physicochemical properties, with only slight fluctuation in expression profile of surface markers compared to untreated cell\u2010derived exosomes. Further investigation revealed that increased expression of p53 might be the predominant reason to promote exosome secretion, potentially through the upregulation of six\u2010transmembrane epithelial antigen of prostate 3 (STEAP3) expression under stress condition. Moreover, we identified that pre\u2010treatment with TMZ\u2010induced exosomes could reduce the sensitivity of C6 cells to TMZ and considered that exosome secretion may serve as a potent TMZ resistance mechanism via enhancing anti\u2010apoptotic ability of GBM cells towards TMZ exposure. Proteomics analysis revealed a strategic mechanism to resist TMZ\u2010induced apoptosis by upregulating exosomal proteins associated with biogenesis, chemoresistance, and therapeutic adaption. This finding revealed a considerable TMZ resistance mechanism and provided a new inspiration for preventing exosome biogenesis to resensitise TMZ cytotoxicity towards GBM.<\/jats:p>","DOI":"10.1002\/jex2.70161","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T21:00:19Z","timestamp":1782853219000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exosome Secretion Drives Chemo\u2010Resistance of Temozolomide in Glioblastoma"],"prefix":"10.1002","volume":"5","author":[{"given":"Wenpei","family":"Li","sequence":"first","affiliation":[{"name":"School of Chemistry and Chemical Engineering School of Life Science School of Interdisciplinary Science Key Laboratory of Molecular Medicine and Biotherapy Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering Beijing Institute of Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoping","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering School of Life Science School of Interdisciplinary Science Key Laboratory of Molecular Medicine and Biotherapy Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering Beijing Institute of Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering School of Life Science School of Interdisciplinary Science Key Laboratory of Molecular Medicine and Biotherapy Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering Beijing Institute of Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi","family":"Li","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering School of Life Science School of Interdisciplinary Science Key Laboratory of Molecular Medicine and Biotherapy Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering Beijing Institute of Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering School of Life Science School of Interdisciplinary Science Key Laboratory of Molecular Medicine and Biotherapy Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering Beijing Institute of Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weimin","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering School of Life Science School of Interdisciplinary Science Key Laboratory of Molecular Medicine and Biotherapy Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering Beijing Institute of Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyu","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering School of Life Science School of Interdisciplinary Science Key Laboratory of Molecular Medicine and Biotherapy Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering Beijing Institute of Technology  Beijing China"},{"name":"School of Medical Engineering School of Interdisciplinary Science; Affiliated Zhuhai People's Hospital Beijing Institute of Technology  Zhuhai P.R. China"},{"name":"Advanced Technology Research Institute Beijing Institute of Technology  Ji'nan China"},{"name":"Beijing Key Laboratory of Mucosal Immunity Vaccines Against Respiratory Viruses Beijing Institute of Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minghui","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering School of Life Science School of Interdisciplinary Science Key Laboratory of Molecular Medicine and Biotherapy Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering Beijing Institute of Technology  Beijing China"},{"name":"School of Medical Engineering School of Interdisciplinary Science; Affiliated Zhuhai People's Hospital Beijing Institute of Technology  Zhuhai P.R. China"},{"name":"Advanced Technology Research Institute Beijing Institute of Technology  Ji'nan China"},{"name":"Beijing Key Laboratory of Mucosal Immunity Vaccines Against Respiratory Viruses Beijing Institute of Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering School of Life Science School of Interdisciplinary Science Key Laboratory of Molecular Medicine and Biotherapy Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering Beijing Institute of Technology  Beijing China"},{"name":"School of Medical Engineering School of Interdisciplinary Science; Affiliated Zhuhai People's Hospital Beijing Institute of Technology  Zhuhai P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6300-4979","authenticated-orcid":false,"given":"Shaobo","family":"Ruan","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering School of Life Science School of Interdisciplinary Science Key Laboratory of Molecular Medicine and Biotherapy Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering Beijing Institute of Technology  Beijing China"},{"name":"School of Medical Engineering School of Interdisciplinary Science; Affiliated Zhuhai People's Hospital Beijing Institute of Technology  Zhuhai P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2023.06.006"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14215435"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2018.1446660"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-018-0025-8"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02316"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/14712598.2017.1305353"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10030512"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2022-004832"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ctd2.341"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2025.04.018"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2019.1648995"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cac2.12414"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2008.168"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.82590"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-013-8620-6"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4477"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-021-00730-1"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.41219"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-022-01671-0"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2021.10.008"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.70127"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21207568"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.3c11958"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2022.05.027"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gendis.2016.04.007"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-024-01209-y"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-019-0450-y"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2008.104"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12672-024-00963-9"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-019-0975-5"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.18133"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-21-0258"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.52570"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-018-0343-6"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202302622"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-024-01669-9"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers17071167"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2015.07.013"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6604856"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.00090"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2017.08.003"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-17717-0"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.70126"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-018-0005-8"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.10.007"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-020-1308-2"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1080\/13880209.2021.1944220"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2024.114168"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-023-03196-y"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2010.04.008"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-015-0459-1"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21623"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1308173110"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-04329-9"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.20892\/j.issn.2095-3941.2019.0228"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-020-00621-9"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.29712"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10025-2"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/noab003"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2023.106880"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.3390\/membranes12050498"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13402-025-01095-0"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41435-025-00327-5"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-024-06695-6"},{"key":"e_1_2_10_66_1","first-page":"249","article-title":"Exosome Mediated Cell\u2010Cell Crosstalk in Tissue Injury and Repair","volume":"73","author":"Yadav A.","year":"2024","journal-title":"Intercellular and Interorganellar Transfer and Communication in Biology and Medicine"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-020-00359-5"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1002\/EXP.20240039"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-41663-2"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2019.03.016"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-022-01509-9"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2017.11.028"}],"container-title":["Journal of Extracellular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jex2.70161","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jex2.70161","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jex2.70161","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T09:23:10Z","timestamp":1784884990000},"score":1,"resource":{"primary":{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/10.1002\/jex2.70161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,30]]},"references-count":71,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/jex2.70161"],"URL":"https:\/\/doi.org\/10.1002\/jex2.70161","archive":["Portico"],"relation":{},"ISSN":["2768-2811","2768-2811"],"issn-type":[{"value":"2768-2811","type":"print"},{"value":"2768-2811","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,30]]},"assertion":[{"value":"2026-03-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70161"}}