{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:51:05Z","timestamp":1792792265227,"version":"4.5.0"},"reference-count":43,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100004411","name":"Heart and Stroke Foundation of Canada","doi-asserted-by":"publisher","award":["G\u201025\u20100040396"],"award-info":[{"award-number":["G\u201025\u20100040396"]}],"id":[{"id":"10.13039\/100004411","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004411","name":"Heart and Stroke Foundation of Canada","doi-asserted-by":"publisher","award":["NI\u201023\u20100034041"],"award-info":[{"award-number":["NI\u201023\u20100034041"]}],"id":[{"id":"10.13039\/100004411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["isevjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Extracellular Bio"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The intestine plays critical roles in nutrient homeostasis and systemic health. The small intestine is the major organ that packages dietary lipids into chylomicrons and secretes them into the mesenteric lymph. Extracellular vesicles (EVs) as a mechanism for cell\u2010to\u2010cell communication often exhibit organ specific characteristics. EV secretion from the intestine into the mesenteric lymph\u2014a unique biofluid\u2014remains unexplored. To characterize gut\u2010derived EVs, Sprague\u2010Dawley rats were surgically implanted with cannula in the mesenteric lymph duct (for lymph collection) and the duodenum (for lipid infusion). Lymph fluid was collected for assessment of EV secretion. EVs in lymph fluids collected before and after lipid infusion were characterized by transmission electron microscopy, nanoparticle tracking analysis, and further analyzed by flow cytometry with antibodies against CD63, CD81, CD9 and apolipoprotein B (ApoB). Lipid infusion increased lymph triglyceride output peaking at 2 h. Lymph fluids contained EVs with diameters in the range of 20 to 300\u00a0nm and the signals of specific EV markers CD63, CD81 and CD9 were significantly elevated following lipid infusion. Depletion of chylomicrons from lymph had differential effects on the percentage and intensity of CD63, CD81 and CD9. In addition, these EVs exhibited distinct patterns in ApoB\n                    <jats:sup>+<\/jats:sup>\n                    particle population, with the median fluorescence intensity of CD63 and CD81 being significantly higher than that of CD9. To conclude, EVs secreted into the mesenteric lymph could rapidly respond to lipid supply. EVs may bind to and co\u2010secrete with chylomicrons. Finally, gut\u2010derived EVs during active lipid absorption may include multiple subtypes with different affinities to chylomicrons. Collectively, these findings highlight the intestine as an EV secretion organ and the mesenteric lymph as an important biofluid harboring EVs, which opens a new venue for future investigation of biological functions of gut\u2010derived EVs.\n                  <\/jats:p>","DOI":"10.1002\/jex2.70170","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T11:13:09Z","timestamp":1784805189000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Secretion of Extracellular Vesicles Into the Mesenteric Lymph During Fasting and Lipid Absorption"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3670-837X","authenticated-orcid":false,"given":"Tianyu","family":"Hang","sequence":"first","affiliation":[{"name":"Department of Anatomy, Physiology and Pharmacology, College of Medicine University of Saskatchewan  Saskatoon Saskatchewan 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Institute  Ottawa Ontario Canada"},{"name":"Department of Cellular and Molecular Medicine, Faculty of Medicine University of Ottawa Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3951-2911","authenticated-orcid":false,"given":"Dylan","family":"Burger","sequence":"additional","affiliation":[{"name":"Kidney Research Centre Ottawa Hospital Research Institute  Ottawa Ontario Canada"},{"name":"Department of Cellular and Molecular Medicine, Faculty of Medicine University of Ottawa Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changting","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Physiology and Pharmacology, College of Medicine University of Saskatchewan  Saskatoon Saskatchewan 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