{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T23:19:41Z","timestamp":1787527181611,"version":"build-2736575974"},"reference-count":84,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000871","name":"Mayo Clinic","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["isevjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Extracellular Bio"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Biofilm\u2010associated staphylococcal infections remain exceptionally difficult to treat due to the presence of resilient staphylococcal biofilms and the limited effectiveness of currently available antibiotics. To combat persistent infections, providers are often forced to use prolonged courses of combination antimicrobial therapies that have significant toxicities with limited effectiveness, leading to increased hospital stays, substantial health care costs and a rise in patient morbidity and mortality.\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    is a dominant cause of recalcitrant biofilms, for which novel non\u2010antibiotic therapeutics are critically needed. Lysins, a class of protein\u2010based antimicrobials, rapidly kill staphylococci, exhibit potent anti\u2010biofilm activity, have a low propensity of resistance development and synergy with antibiotics. To address this unmet need, we evaluate an extracellular vesicle (EV)\u2010based delivery platform for the engineered anti\u2010staphylococcal lysin LYSG101, designed to improve stability and localization at sites of infection. Initial proof\u2010of\u2010concept work is provided here, demonstrating that human serum\u2010derived EVs can be loaded with LYSG101 to exert a potent in vitro antimicrobial effect against both planktonic and biofilm forms of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    . EV\u2010mediated delivery achieved activity equivalent to free lysin, with additional translational advantages including stability and the potential for sustained intra\u2010articular retention. This work supports further development of the EV\u2010mediated lysin delivery as a broadly applicable antimicrobial platform, with potential future applications in biofilm\u2010associated infections, including prosthetic joint infection (PJI).\n                  <\/jats:p>","DOI":"10.1002\/jex2.70174","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T06:46:07Z","timestamp":1786085167000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["EV\u2010LYSG101: Extracellular Vesicles Loaded With Lysin LYSG101 for Potent Anti\u2010Staphylococcal Activity"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0861-9310","authenticated-orcid":false,"given":"Dominique B.","family":"Hoelzinger","sequence":"first","affiliation":[{"name":"Department of Regenerative Biotherapeutics Mayo Clinic  Scottsdale Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ava M. Koehler de","family":"Celaya","sequence":"additional","affiliation":[{"name":"Department of Regenerative Biotherapeutics Mayo Clinic  Scottsdale Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheryl E.","family":"Myers","sequence":"additional","affiliation":[{"name":"Department of Regenerative Biotherapeutics Mayo Clinic  Scottsdale Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natasha P.","family":"Dyal","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Mayo Clinic  Phoenix Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher F.","family":"Saling","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Mayo Clinic  Phoenix Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond","family":"Schuch","sequence":"additional","affiliation":[{"name":"Precisio Biotix Therapeutics, Inc.  Dover Delaware USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gina A.","family":"Suh","sequence":"additional","affiliation":[{"name":"Division of Public Health, Infectious Diseases, and Occupational Medicine, Department of Medicine Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01515-21"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.00069-24"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.20517\/evcna.2024.21"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12048"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsml\/uqab003"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph14040356"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2025.2562634"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00285-16"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jex2.59"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.817844"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2021.550853"},{"key":"e_1_2_9_13_1","doi-asserted-by":"crossref","unstructured":"Ferry T. 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