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Here, we present a scalable manufacturing strategy for nanoalgosomes, EVs derived from the microalga\n                    <jats:italic>Tetraselmis chuii<\/jats:italic>\n                    . The process integrates upstream cultivation under controlled conditions with downstream isolation and purification via continuous tangential flow filtration (TFF). Critical TFF parameters\u2014cross\u2010flow velocity, shear rate, permeate flux, and transmembrane pressure\u2014were investigated to evaluatetheir impact on process behaviour, extracellular vesicle recovery, and functional integrity. Following the MISEV2018 guidelines established in our previous baseline characterizations, the isolated nanoalgosomes were verified by a quality control, including biochemical and biophysical characterization, confirming the robustness of the platform. Overall, this work provides a practical processing framework for the scientific community dealing with large\u2010volume EV bioprocessing, offering an informative baseline for the scalable production of microalgae\u2010derived extracellular vesicles.\n                  <\/jats:p>","DOI":"10.1002\/jex2.70185","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T07:56:12Z","timestamp":1789113372000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Scalable and Controlled Production of Microalgae\u2010derived Extracellular Vesicles Using a Differential Tangential Flow Filtration Protocol"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4903-4143","authenticated-orcid":false,"given":"Angela","family":"Paterna","sequence":"first","affiliation":[{"name":"Institute of Biophysics (IBF) and Cell\u2010Tech HUB National Research Council of Italy (CNR)  Palermo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estella","family":"Rao","sequence":"additional","affiliation":[{"name":"Institute of Biophysics (IBF) and Cell\u2010Tech HUB National Research Council of Italy (CNR)  Palermo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Gargano","sequence":"additional","affiliation":[{"name":"Institute of Biophysics (IBF) and Cell\u2010Tech HUB National Research Council of Italy (CNR)  Palermo Italy"},{"name":"Cell\u2010Tech HUB and Institute for Biomedical Research and Innovation (IRIB) National Research Council of Italy (CNR)  Palermo Italy"},{"name":"EVE Biofactory s.r.l.  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