{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T06:03:31Z","timestamp":1789970611986,"version":"4.0.1"},"reference-count":71,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["isevjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Extracellular Bio"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Recombinant human nerve growth factor (NGF) is used to treat patients with impaired corneal wound healing. NGF exerts its effects through complex signaling processes initiated by the binding of one of two cell membrane receptors\u2014TrkA and p75NTR. While the intracellular signaling pathways have been studied extensively, how NGF mediates intercellular signaling to coordinate wound healing is less understood. Here, we demonstrate that extracellular vesicles (EVs) produced by the corneal epithelium in response to exogenous NGF negatively affect the viability, proliferation, and migration of corneal epithelial cells. We found that EVs produced in response to NGF include an over\u2010enrichment in miR\u201034a\u20103p, while the same miRNA is under\u2010expressed in the cell lysate after NGF treatment. Conversely, treatment with a miR\u201034a\u20103p antagonist, miR34a\u2010i, induces a significant upregulation of endogenous NGF, suggesting a potential feedback pathway between miR\u201034a\u20103p and NGF. Furthermore, media conditioned with EVs produced in response to miR34a\u2010i treatment significantly enhances the proliferative response in the corneal epithelium. We identified bex3\u2014encoding NGF receptor associated protein 1\u2014as a potential target of miR\u201034a\u20103p in the NGF signaling pathway. These data highlight a potential intercellular negative feedback pathway induced by NGF treatment in the corneal epithelium.<\/jats:p>","DOI":"10.1002\/jex2.70192","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T05:25:36Z","timestamp":1789709136000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nerve Growth Factor Treatment and the Corneal Epithelium: Extracellular Vesicle\u2010Mediated Signaling"],"prefix":"10.1002","volume":"5","author":[{"given":"Levi N.","family":"Kanu","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Schepens Eye Research Institute of Mass Eye and Ear Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Chatterjee","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Schepens Eye Research Institute of Mass Eye and Ear Harvard 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