{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T00:15:37Z","timestamp":1700352937647},"reference-count":27,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2002,10,29]],"date-time":"2002-10-29T00:00:00Z","timestamp":1035849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Exp. Zool."],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to compare levels of stress proteins in four <jats:italic>Trichinella<\/jats:italic> species when exposed to different stressors. Heat shock protein (HSP) 60, 70 and 90 responses were evaluated in infective larvae (L<jats:sub>1<\/jats:sub>) of four classic <jats:italic>Trichinella<\/jats:italic> species following exposure to oxidative, anthelminthic and thermal stress. Larvae of <jats:italic>T. nativa<\/jats:italic>, <jats:italic>T nelsoni<\/jats:italic>, <jats:italic>T. pseudospiralis<\/jats:italic> and <jats:italic>T. spiralis<\/jats:italic> were exposed to peroxide shock (0.2%, 1%, or 2% H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>for 2h), high temperatures (40\u00b0C or 45\u00b0C for 2h), or 0.1\u00b5g\/ml of the benzimidazole anthelminthics: mebendazole (MBZ), albendazole (ALB) or thiabendazole (TBZ) for 4h. Following exposures, the L<jats:sub>1<\/jats:sub> were tested for induced morphological changes. Those observed were: (i) no change (in all species exposed to 40\u00b0 C) (ii) aberrant forms (in all species exposed to anthelminthics, in <jats:italic>T. nativa<\/jats:italic>, <jats:italic>T. nelsoni<\/jats:italic> and <jats:italic>T. spiralis<\/jats:italic> exposed to 45\u00b0C, and in <jats:italic>T. spiralis<\/jats:italic> and <jats:italic>T. nelsoni<\/jats:italic> exposed to 0.2% H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) and (iii) severe degradation or death (in <jats:italic>T. nativa<\/jats:italic> and <jats:italic>T. pseudospiralis<\/jats:italic> exposed to 0.2% H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, and in all species at 1% and 2% H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>). In Western blot analyses, L<jats:sub>1<\/jats:sub> proteins were probed with monoclonal antibodies (mAbs) specific for the three HSPs. Greater changes in HSP levels occurred following H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> exposure than with other stresses in all <jats:italic>Trichinella<\/jats:italic> species, while accumulation of a 50 kDa HSP was only observed in <jats:italic>T. spiralis<\/jats:italic> and <jats:italic>T. pseudospiralis<\/jats:italic>. Anthelminthic stress only caused decreased HSP levels in <jats:italic>T. nativa<\/jats:italic>. Thermal stress caused no significant changes in the HSP response of any species. It is suggested that other stress proteins (e.g., glucose\u2010regulated proteins) may be involved in adaptation to thermal stress. <jats:italic>J. Exp. Zool. 293:664\u2013674, 2002.<\/jats:italic> \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jez.10170","type":"journal-article","created":{"date-parts":[[2002,10,31]],"date-time":"2002-10-31T18:21:48Z","timestamp":1036088508000},"page":"664-674","source":"Crossref","is-referenced-by-count":4,"title":["Oxidative, heat and anthelminthic stress responses in four species of <i>Trichinella<\/i>: comparative study"],"prefix":"10.1002","volume":"293","author":[{"given":"Javier","family":"Martinez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Perez\u2010Serrano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.E.","family":"Bernadina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filomena","family":"Rodriguez\u2010Caabeiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,10,29]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(52)90014-3"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4613-3578-8"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"7901","DOI":"10.1016\/S0021-9258(18)47653-3","article-title":"Differences in the regulation of messenger RNA for housekeeping and specialized\u2010cell ferritin. 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