{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T02:54:22Z","timestamp":1771642462387,"version":"3.50.1"},"reference-count":12,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,6,1]],"date-time":"2005-06-01T00:00:00Z","timestamp":1117584000000},"content-version":"vor","delay-in-days":9070,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Exp. Zool."],"published-print":{"date-parts":[[1980,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In 82 wild\u2010caught <jats:italic>Crocodylus porosus<\/jats:italic>, levels of NADH\u2010MetHb reductase and GSH seem adequate to maintain hemoglobin in its reduced functional state. Studies of <jats:italic>C. porosus<\/jats:italic> erythrocytes in vitro show reduction of metHb in the presence of lactate, glucose and plasma, but not pyruvate.<\/jats:p><jats:p>These findings, together with recent data which show low metHb in a variety of reptiles, cast doubt on the accepted view that high levels of MetHb are typical of healthy reptiles.<\/jats:p><jats:p>One explanation for the sharp contrast between earlier and more recent data could be technical. We found low metHb in <jats:italic>Crocodylus johnstoni, Chelodina longicollis<\/jats:italic> and <jats:italic>Sphenomorphus quoyi<\/jats:italic>. However, high and variable values reminiscent of many of the earlier data were obtained by omitting final centrifugation prior to spectrophotometry. Interestingly, this step is not part of the standard clinical method but is necessary in analyses of blood with nucleated red cells. These observations suggest that high metHb may not be typical of reptiles after all.<\/jats:p>","DOI":"10.1002\/jez.1402130221","type":"journal-article","created":{"date-parts":[[2005,6,12]],"date-time":"2005-06-12T00:31:24Z","timestamp":1118536284000},"page":"305-308","source":"Crossref","is-referenced-by-count":19,"title":["Methemoglobin reduction in crocodile blood: Are high levels of MetHb typical of healthy reptiles?"],"prefix":"10.1002","volume":"213","author":[{"given":"Margie","family":"Gruca","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gordon C.","family":"Grigg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"265","article-title":"Methaemoglobin and its reduction in nucleated erythrocytes from reptiles and birds","volume":"57","author":"Board P. G.","year":"1977","journal-title":"Comp. Biochem. Physiol."},{"key":"e_1_2_1_3_1","first-page":"1","volume-title":"Biology of the Reptilia","author":"Dessauer H. C.","year":"1970"},{"key":"e_1_2_1_4_1","first-page":"49","volume-title":"Hemoglobin, Its Precursors and Metabolites","author":"Dubowski K. M.","year":"1960"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1016\/S0021-9258(18)73873-8","article-title":"Microdetermination of oxyhemoglobin, methemoglobin and sulfhemoglobin in a single example of blood","volume":"126","author":"Evelyn K. A.","year":"1938","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_6_1","unstructured":"Grigg G. C.(1980)Water and electrolyte homeostatis inCrocodylus porosusalong a salinity gradient. (In preparation)."},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","unstructured":"Grigg G. C. andM.Cairncross(1980)Respiratory properties of the blood ofCrocodylus porosus. Submitted to Respiration Physiology.","DOI":"10.1016\/0034-5687(80)90083-3"},{"key":"e_1_2_1_8_1","unstructured":"Gruca M. G. C.Grigg andJ.Gupta(1980)Erythrocyte metabolism inCrocodylus porosus. 1.Glycolytic enzymes. (In preparation)"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0010-406X(69)91798-8"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.103.2674.406"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)97676-3"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/2041098a0"},{"key":"e_1_2_1_13_1","first-page":"225","volume-title":"Biology of the Reptilia","author":"Wood S. C.","year":"1976"}],"container-title":["Journal of Experimental Zoology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjez.1402130221","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jez.1402130221","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T12:14:22Z","timestamp":1699791262000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jez.1402130221"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,8]]},"references-count":12,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1980,8]]}},"alternative-id":["10.1002\/jez.1402130221"],"URL":"https:\/\/doi.org\/10.1002\/jez.1402130221","archive":["Portico"],"relation":{},"ISSN":["0022-104X","1097-010X"],"issn-type":[{"value":"0022-104X","type":"print"},{"value":"1097-010X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1980,8]]}}}