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During 48 h of culture under these condition, embryos grew from egg cylinders to complex structures with neural folds and other organ rudiments. Most embryos had beating hearts, numerous pigmented red blood cells, and a fused chorioallantoic placenta. The results suggest that factors in ascites fluid support differentiation and growth of mouse embryos. Addition of bovine serum albumin was also beneficial for development. Differential sister chromatid staining of cultured embryos showed that ascites fluid increased the rate of clel proliferation as compared with medium containing bovine serum albumin alone, or bovine serum albumin and fetal calf serum. These results with abundant, and readily available supplements may facilitate certain studies of mouse embryo development.<\/jats:p>","DOI":"10.1002\/jez.1402310309","type":"journal-article","created":{"date-parts":[[2005,6,11]],"date-time":"2005-06-11T12:51:32Z","timestamp":1118494292000},"page":"355-362","source":"Crossref","is-referenced-by-count":2,"title":["Ascites tumor fluid and bovine serum albumin as supplements in presomite mouse embryo cultures"],"prefix":"10.1002","volume":"231","author":[{"given":"Juanito J.","family":"Meneses","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger A.","family":"Pedersen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,25]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-154-39690"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(63)90093-4"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/238398a0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7123220"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4827(75)80041-3"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/tera.1420020302"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402240210"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.6.3524"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/263594a0"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.140.3568.802"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4827(63)80014-2"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0530335"},{"key":"e_1_2_1_14_1","first-page":"471","volume-title":"Differentiation and Control of Malignancy of Tumor Cells","author":"Hozumi M.","year":"1974"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(80)90394-2"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/tera.1420270116"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.147.3.923"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2964(72)90044-8"},{"key":"e_1_2_1_19_1","first-page":"K43","article-title":"Chalone\u2010like inhibition of Ehrlich ascites cell proliferation in vitro by an ultrafiltrate obtained from the ascitic fluid","volume":"36","author":"Lehmann W.","year":"1977","journal-title":"Act. 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