{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,12,23]],"date-time":"2023-12-23T22:50:03Z","timestamp":1703371803842},"reference-count":70,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,6,1]],"date-time":"2005-06-01T00:00:00Z","timestamp":1117584000000},"content-version":"vor","delay-in-days":6514,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Exp. Zool."],"published-print":{"date-parts":[[1987,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have studied the ability of fertilized eggs of <jats:italic>Ilyanassa obsoleta<\/jats:italic> to undergo polar lobe formation and cytokinesis in the presence of Ca<jats:sup>2+<\/jats:sup> antagonists (Ca<jats:sup>2+<\/jats:sup> channel blockers, Ca<jats:sup>2+<\/jats:sup> uptake inhibitors). Earlier work had suggested little need for exogenous Ca<jats:sup>2+<\/jats:sup> during these cellular shape changes. Again it appears that exogenous Ca<jats:sup>2+<\/jats:sup> probably is not required, based on cell ability to undergo the shape changes with no, or only minor, delay in the presence of 50 mM La<jats:sup>3+<\/jats:sup> at pH 6.5, 10 mM concentrations of Ni<jats:sup>2+<\/jats:sup> or Co<jats:sup>2+<\/jats:sup>, 1 mM Cd<jats:sup>2+<\/jats:sup>, and 100 \u03bcM concentrations of Mn<jats:sup>2+<\/jats:sup>, papaverine, verapamil, D600, or diltiazem. In nominally Ca<jats:sup>2+<\/jats:sup> \u2010free seawater (containing \u223c 10 \u03bcM Ca<jats:sup>2+<\/jats:sup>) (CFSW), there still is no effect of Cd<jats:sup>2+<\/jats:sup> (up to 100 \u03bcM), Ni<jats:sup>2+<\/jats:sup>, Co<jats:sup>2+<\/jats:sup>, Mn<jats:sup>2+<\/jats:sup>, or diltiazem; however, papaverine, verapamil, and D600 in CFSW cause longer delays in the shape changes than they do in the presence of normal levels of Ca<jats:sup>2+<\/jats:sup> (SW). In 10\u201350 \u03bcM nifedipine, shape changes are progressively delayed to the same extent in both SW and CFSW, but more so in CFSW at concentrations above 50 \u03bcM nifedipine.<\/jats:p><jats:p>Among calmodulin antagonists, trifluoperazine up to 100 \u03bcM was without effect, but chlorpromazine at 25\u2013100 \u03bcM and calmidazolium at 50\u2013100 \u03bcM caused substantial, concentration\u2010dependent delays in the starting times for the shape changes. Methylxanthines caused a substantial <jats:italic>speed\u2010up<\/jats:italic> in the starting times for both polar lobe formation and cytokinesis. The most effective of these, caffeine, at optimal concentrations of 0.7\u201310 mM in SW or CFSW caused shape changes to occur 12\u201315 min <jats:italic>earlier<\/jats:italic> than in controls undergoing a normal 50\u2010min cycle. Caffeine is known to cause release of Ca<jats:sup>2+<\/jats:sup> from muscle sarcoplasmic reticulum. A putative antagonist of intracellular Ca<jats:sup>2+<\/jats:sup> mobilization, TMB\u20108, significantly inhibited the shape changes of the <jats:italic>Ilyanassa<\/jats:italic> cells, whereas a variety of inhibitors of exogenous Ca<jats:sup>2+<\/jats:sup> uptake noted above did not inhibit. We conclude that Ca<jats:sup>2+<\/jats:sup> may be necessary for polar lobe formation and cytokinesis in <jats:italic>Ilyanassa<\/jats:italic> cells, but that it may be released from intracellular, sequestered stores rather than derived from exogenous sources.<\/jats:p>","DOI":"10.1002\/jez.1402430210","type":"journal-article","created":{"date-parts":[[2005,6,11]],"date-time":"2005-06-11T03:02:24Z","timestamp":1118458944000},"page":"245-258","source":"Crossref","is-referenced-by-count":11,"title":["Effects of calcium antagonists, calmodulin antagonists, and methylated xanthines on polar lobe formation and cytokinesis in fertilized eggs of <i>Ilyanassa obsoleta<\/i>"],"prefix":"10.1002","volume":"243","author":[{"given":"Gary W.","family":"Conrad","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula V.","family":"Glackin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robyn A.","family":"Hay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto R.","family":"Patron","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(84)90258-6"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.20.6388"},{"key":"e_1_2_1_4_1","first-page":"598","article-title":"\u03b1\u2010Adrenergic blockade and inhibition of A23187 mediated Ca2+ uptake by the calcium antagonist verapamil in rat liver cells","volume":"15","author":"Blackmore P. F.","year":"1979","journal-title":"Mol. Pharmacol."},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198212233072605"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"5384","DOI":"10.1016\/S0021-9258(18)91018-5","article-title":"Direct photo\u2010affinity labeling of the high affinity nitrendipine\u2010binding site in subcellular membrane fractions isolated from canine myocardium","volume":"259","author":"Campbell K. P.","year":"1984","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.5.1549"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-198406004-00009"},{"key":"e_1_2_1_9_1","volume-title":"Formulae and Methods VI of the Marine Biological Laboratory Chemical Room","author":"Cavanaugh G. M.","year":"1975"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(85)90261-4"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(74)90058-X"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(80)90058-5"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-781240-3.50020-2"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/01688170.1986.10510196"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(77)90367-0"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1986.sp016132"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01870203"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1983.tb08143.x"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(84)90367-8"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(82)91089-0"},{"key":"e_1_2_1_21_1","unstructured":"Fleckenstein A.(1983) History of calcium antagonists. In: Calcium Channel\u2010Blocking Drugs."},{"key":"e_1_2_1_21_2","first-page":"I\u20103","volume":"52","journal-title":"Circ. Res."},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402290114"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.1984.sp002812"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(82)91221-4"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.11.3656"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bb.08.060179.002125"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ne.04.030181.000441"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(83)90084-X"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(83)90164-1"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.2170\/jjphysiol.32.609"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.8.2582"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(81)90483-8"},{"key":"e_1_2_1_33_1","first-page":"8","article-title":"Characteristics of the binding of [3H]nitrendipine to rabbit ventricular membranes: Modification by other Ca++ channel antagonists and by the Ca++ channel agonist Bay K8644","volume":"231","author":"Janis R. A.","year":"1984","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_1_34_1","first-page":"21","volume-title":"Calcium Entry Blockers and Tissue Protection","author":"Janis R. A.","year":"1985"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.5.1460"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(83)90332-9"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(83)90258-0"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(84)91555-9"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/302790a0"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4612-5172-9_3"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(84)80241-7"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(83)90404-6"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(84)91619-X"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/304635a0"},{"key":"e_1_2_1_45_1","doi-asserted-by":"crossref","first-page":"8378","DOI":"10.1016\/S0021-9258(18)43501-6","article-title":"Inhibitory action of chlorpromazine, dibucaine, and other phospholipid\u2010interacting drugs on calcium\u2010activated, phospholipid\u2010dependent protein kinase","volume":"255","author":"Mori T.","year":"1980","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(84)90037-6"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.3.860"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402400206"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.2307\/1541159"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(84)90002-0"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(82)92005-8"},{"key":"e_1_2_1_52_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2000109"},{"key":"e_1_2_1_53_1","first-page":"123","article-title":"Temperature dependence of verapamil action","volume":"1","author":"Piper H. M.","year":"1985","journal-title":"Basic. Res. Cardiol. [Suppl.]"},{"key":"e_1_2_1_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(85)90463-2"},{"key":"e_1_2_1_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(83)80015-5"},{"key":"e_1_2_1_56_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2422760"},{"key":"e_1_2_1_57_1","first-page":"213","volume-title":"Mechanism and Control of Animal Fertilization","author":"Shen S. S.","year":"1983"},{"key":"e_1_2_1_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(84)80928-X"},{"key":"e_1_2_1_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(83)90146-7"},{"key":"e_1_2_1_60_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6322304"},{"key":"e_1_2_1_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(85)91812-1"},{"key":"e_1_2_1_62_1","first-page":"121","volume-title":"Calcium Blockers: Mechanisms of Action and Clinical Applications","author":"Triggle D. J.","year":"1982"},{"key":"e_1_2_1_63_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.45.030183.002013"},{"key":"e_1_2_1_64_1","first-page":"B\u2010122","volume-title":"Handbook of Chemistry and Physics","author":"Weast R. C.","year":"1976"},{"key":"e_1_2_1_65_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pa.14.040174.002015"},{"key":"e_1_2_1_66_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-114-1-116"},{"key":"e_1_2_1_67_1","first-page":"267","volume-title":"The Physiology of Excitable Cells (Vol. 5 of Neurology and Neurobiology)","author":"Yoshida S.","year":"1983"},{"key":"e_1_2_1_68_1","doi-asserted-by":"publisher","DOI":"10.1247\/csf.10.29"},{"key":"e_1_2_1_69_1","doi-asserted-by":"crossref","first-page":"387","DOI":"10.2220\/biomedres.6.387","article-title":"Cyclic changes in intracellular free calcium levels associated with cleavage cycles in echnoderm and Medaka eggs","volume":"6","author":"Yoshimoto Y.","year":"1985","journal-title":"Biomed. Res."},{"key":"e_1_2_1_70_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1984.tb08300.x"}],"container-title":["Journal of Experimental Zoology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjez.1402430210","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jez.1402430210","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T22:50:39Z","timestamp":1697842239000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jez.1402430210"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,8]]},"references-count":70,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1987,8]]}},"alternative-id":["10.1002\/jez.1402430210"],"URL":"https:\/\/doi.org\/10.1002\/jez.1402430210","archive":["Portico"],"relation":{},"ISSN":["0022-104X","1097-010X"],"issn-type":[{"value":"0022-104X","type":"print"},{"value":"1097-010X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,8]]}}}