{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T18:32:25Z","timestamp":1751481145188},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,6,1]],"date-time":"2005-06-01T00:00:00Z","timestamp":1117584000000},"content-version":"vor","delay-in-days":6483,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Exp. Zool."],"published-print":{"date-parts":[[1987,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effect of several solubilized monosaccharides on epidermal cell migration from skin explants of the adult newt was examined. The ability of epidermal cells to migrate on substrates coated with these same sugars or with wheat germ agglutinin (WGA) was also determined. Adding 0.05 M Nacetyl\u2010glucosamine (GlcNAc) to the medium inhibited epidermal cell migration in dishes coated with either type I collagen or fibrinogen. The same concentration of fucose, galactose, or mannose had no effect. In contrast to type I collagen, which supported considerable migration when dried onto the bottom of plastic dishes, epidermal cells were unable to migrate on dishes coated similarly with WGA, mucin (a protein high in sialic acid residues), or bovine serum albumin (BSA) conjugated to galactose, mannose, or GlcNAc. Red blood cell (RBC) binding assays showed that drying WGA onto plastic did not destroy its GlcNAc binding sites\u2014nor did it damage the GlcNAc residues of BSA\u2010GlcNAc. The RBC assay also verified that for both these proteins, substrates with distinctly different cell binding capacities had been tested in the migration experiments. In dishes coated with either WGA or BSA\u2010GlcNAc, red cells bound to dish bottoms in a GlcNAc\u2010specific manner right up to the margins of explants. Other control experiments showed that the failure of migration in WGA\u2010 and BSA\u2010GlcNAc\u2010coated dishes could not be explained by competition between adsorbed and desorbing protein for cell surface receptors. This work shows that adhesive bonds between epidermal cell surface GlcNAc and a GlcNAC\u2010specific lectin on the substrate are not by themselves adequate to support cell migration. Nor is GlcNAc, sialic acid, galactose, or mannose alone on the substrate sufficient. In conjunction with our earlier work (Donaldson and Mahan: <jats:italic>J. Exp. Zool., 231<\/jats:italic>:211\u2013219, '84; Donaldson, Mahan, Hasty, McCarthy, and Furcht: <jats:italic>J. Cell. Biol., 101<\/jats:italic>: 73\u201378, '85), these observations suggest that factors other than carbohydrate content or capacity to act as a lectin determine whether a given extracellular protein will support migration.<\/jats:p>","DOI":"10.1002\/jez.1402430313","type":"journal-article","created":{"date-parts":[[2005,6,11]],"date-time":"2005-06-11T10:10:21Z","timestamp":1118484621000},"page":"461-471","source":"Crossref","is-referenced-by-count":2,"title":["Role of carbohydrates in cell\u2010substrate interactions during newt epidermal cell migration"],"prefix":"10.1002","volume":"243","author":[{"given":"Karen Davenport","family":"Atnip","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James T.","family":"Mahan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald J.","family":"Donaldson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470122860.ch3"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.88.1.138"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.102.3.688"},{"key":"e_1_2_1_5_1","first-page":"387","article-title":"The role of tissue specificity in the healing of epithelial wounds","volume":"121","author":"Chiakulus J.","year":"1952","journal-title":"J. Exp. Zool."},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.93.2.402"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.99.4.1416"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1242\/jcs.62.1.117","article-title":"Fibrinogen and fibronectin as substrates for epidermal cell migration during wound closure","volume":"62","author":"Donaldson D. J.","year":"1983","journal-title":"J. Cell Sci."},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402310206"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402390209"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.101.1.73"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1242\/jcs.57.1.15","article-title":"Epidermal cell migration on collagen and collagen\u2010derived peptides","volume":"57","author":"Donaldson D. J.","year":"1982","journal-title":"J. Cell Sci."},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"6856","DOI":"10.1016\/S0021-9258(18)34508-3","article-title":"Carbohydrate structure of hamster plasma fibronectin. Evidence for chemical diversity between cellular and plasma fibronectins","volume":"257","author":"Fukuda M.","year":"1982","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.10.5129"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(69)90345-6"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)43473-9"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0045-6039(83)90014-3"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.103.3.1055"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02796429"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.88.1.127"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"7940","DOI":"10.1016\/S0021-9258(17)34462-9","article-title":"Adhesion of chicken hepatocytes to polyacrylamide gels derivatized with N\u2010Acetylglucosamine","volume":"253","author":"Schnaar R. L.","year":"1978","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00222492"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)94398-X"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.99.4.1535"},{"key":"e_1_2_1_25_1","doi-asserted-by":"crossref","first-page":"6860","DOI":"10.1016\/S0021-9258(19)62695-5","article-title":"Implications of distinct inhibitory effects of N\u2010Acetylglucosamine on glucose uptake by an isolated perfusion system incorporating erythrocytes with livers from fed and 48\u2010hour fasted rats","volume":"261","author":"Sukalski K. A.","year":"1986","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_26_1","doi-asserted-by":"crossref","first-page":"9704","DOI":"10.1016\/S0021-9258(18)34130-9","article-title":"Carbohydrate structure of human fibrinogen. Use of 300\u2010MHz H\u2010NMR to characterize glycosidase\u2010treated glycopeptides","volume":"257","author":"Townsend R. R.","year":"1982","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_27_1","doi-asserted-by":"crossref","first-page":"10830","DOI":"10.1016\/S0021-9258(19)86596-1","article-title":"Adhesion of hepatocytes to immobilized sugars. A threshold phenomenon","volume":"254","author":"Weigel P. H.","year":"1979","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.103.2.361"}],"container-title":["Journal of Experimental Zoology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjez.1402430313","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jez.1402430313","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T07:37:42Z","timestamp":1697873862000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jez.1402430313"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,9]]},"references-count":27,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1987,9]]}},"alternative-id":["10.1002\/jez.1402430313"],"URL":"https:\/\/doi.org\/10.1002\/jez.1402430313","archive":["Portico"],"relation":{},"ISSN":["0022-104X","1097-010X"],"issn-type":[{"value":"0022-104X","type":"print"},{"value":"1097-010X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,9]]}}}