{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T17:08:08Z","timestamp":1698340088344},"reference-count":12,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,5,12]],"date-time":"2005-05-12T00:00:00Z","timestamp":1115856000000},"content-version":"vor","delay-in-days":6250,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Exp. Zool."],"published-print":{"date-parts":[[1988,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previously, increases in ciliary length have only been obtained through genetic mutation in <jats:italic>Chlamydomonas<\/jats:italic> or by incubation of swimming echinoderm blastulae in trypsin or elastase. We have found that the phenotypic switch from short to long cilia on sand dollar blastulae can also be effected by incubation in theophylline. Cilia detached from control blastulae have a mean length of 21 \u00b1 7 \u03bcm with 10% of the cilia being &gt; 30 \u03bcm. Upon incubation in 10 mM theophylline additional long cilia appeared after 10 hours and by 24\u201332 hours 1\/2\u20133\/4 of the embryo was covered with long cilia. The percentage of long cilia increased to 65% with a mean length of 40.0 \u00b1 17.6 \u03bcm. Incubation in other methylxanthines, such as aminophylline, caffeine, or isobutylmethylxanthine, inhibited development but had no effect on ciliary length distribution. Dibutyryl cAMP, 8\u2010bromoad\u2010enosine, and calcium ionophore also had no effect on ciliary length. Cyclic AMP levels were measured and showed only slight differences among controls and embryos incubated in trypsin, caffeine, or theophylline. These data suggest that theophylline may be altering ciliary length control through some mechanism other than elevations in cAMP.<\/jats:p>","DOI":"10.1002\/jez.1402460104","type":"journal-article","created":{"date-parts":[[2005,6,11]],"date-time":"2005-06-11T03:11:26Z","timestamp":1118459486000},"page":"17-22","source":"Crossref","is-referenced-by-count":3,"title":["Effects of theophylline on expression of the long cilia phenotype in sand dollar blastulae"],"prefix":"10.1002","volume":"246","author":[{"given":"Mary Ann","family":"Riederer\u2010Henderson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,5,12]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1242\/jcs.37.1.205","article-title":"Kinetics of the regeneration of sea urchin cilia. II. Regeneration of animalized cilia","volume":"37","author":"Burns R.","year":"1979","journal-title":"J. Cell Sci."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0309-1651(78)90035-8"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(73)90583-1"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.68.3.440"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4827(75)80030-9"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(79)90041-1"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402400306"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2422760"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.64.1.367"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(77)90301-3"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(84)90158-1"}],"container-title":["Journal of Experimental Zoology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjez.1402460104","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jez.1402460104","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T17:07:12Z","timestamp":1697908032000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jez.1402460104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,4]]},"references-count":12,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1988,4]]}},"alternative-id":["10.1002\/jez.1402460104"],"URL":"https:\/\/doi.org\/10.1002\/jez.1402460104","archive":["Portico"],"relation":{},"ISSN":["0022-104X","1097-010X"],"issn-type":[{"value":"0022-104X","type":"print"},{"value":"1097-010X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,4]]}}}