{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T10:27:30Z","timestamp":1789208850483,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,4,26]],"date-time":"2005-04-26T00:00:00Z","timestamp":1114473600000},"content-version":"vor","delay-in-days":4682,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Exp. Zool."],"published-print":{"date-parts":[[1992,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The objective of this study was to identify, characterize, and examine oviductal secretory proteins (OSP) synthesized de novo by whole oviduct (WO), ampulla (A), and isthmic (I) tissue from ovariectomized (OVX), corn oil (CO)\u2010, estrogen (E)\u2010, progesterone (P)\u2010, and E + P\u2010treated gilts. Oviducts were collected from OVX gilts after CO, E, P, or E + P treatment for 11 consecutive days and tissue was incubated with <jats:sup>3<\/jats:sup>H\u2010leucine (<jats:sup>3<\/jats:sup>H\u2010leu). Rates of <jats:sup>3<\/jats:sup>H\u2010leu incorporation into nondialyzable macromolecules by WO explants were greater (<jats:italic>P<\/jats:italic> &lt; 0.01) with E\u2010 compared to CO\u2010, P\u2010, or E + P\u2010treated gilts and greater (<jats:italic>P<\/jats:italic> &lt; 0.05) by A explants with E\u2010 compared to CO\u2010, P\u2010, or E + P\u2010treated gilts. An effect of location was noted, with A having a greater (<jats:italic>P<\/jats:italic> &lt; 0.01) rate of incorporation than WO or I. Conditioned culture medium was analyzed by one (1D)\u2010 and two\u2010dimensional (2D) sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and fluorography. Analyses by 1D\u2010SDS\u2010PAGE revealed three major E\u2010dependent bands (335,000, 100,000, and 80,000 M<jats:sub>r<\/jats:sub>) in WO and A, and one (335,000 M<jats:sub>r<\/jats:sub>) in the I. A 20,000 M<jats:sub>r<\/jats:sub> band found in A was inhibited by E, while a 60,000 M<jats:sub>r<\/jats:sub> band found in the A was induced by P. Analyses by 2D\u2010SDS\u2010PAGE resolved major E\u2010dependent bands 2 (100,000 M<jats:sub>r<\/jats:sub>) and 3 (80,000 M<jats:sub>r<\/jats:sub>) into basic and acidic 100,000 M<jats:sub>r<\/jats:sub> proteins and a 75,000\u201385,000 M<jats:sub>r<\/jats:sub> protein (pI &lt; 4), respectively, found in WO and A, but not in I. A basic 20,000 M<jats:sub>r<\/jats:sub> protein and an acidic 45,000 M<jats:sub>r<\/jats:sub> complex, both found in A, were inhibited by E. Gel filtration of culture medium revealed a high M<jats:sub>r<\/jats:sub> fraction (&gt; 2 \u00d7 10<jats:sup>6<\/jats:sup>) that was induced by E and was 6.8\u2010fold greater in medium from A than from I. This study clearly demonstrates that 1) WO and A tissue from E\u2010treated gilts de novo synthesize and secrete three major proteins (basic 100,000, acidic 100,000, and 75,000\u201385,000 M<jats:sub>r<\/jats:sub>); 2) these E\u2010dependent proteins are not found in I or with other treatment; 3) several protein complexes synthesized by A are inhibited by E treatment; and 4) a high M<jats:sub>r<\/jats:sub> fraction, produced primarily in the A, is induced or amplified by E. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jez.1402620409","type":"journal-article","created":{"date-parts":[[2005,6,11]],"date-time":"2005-06-11T13:00:16Z","timestamp":1118494816000},"page":"426-435","source":"Crossref","is-referenced-by-count":40,"title":["In vitro synthesis of oviductal secretory proteins by estrogen\u2010treated ovariectomized gilts"],"prefix":"10.1002","volume":"262","author":[{"given":"W. 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