{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T06:43:06Z","timestamp":1698734586326},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,5,14]],"date-time":"2012-05-14T00:00:00Z","timestamp":1336953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Exp. Zool."],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Little is known about the reproductive biology of <jats:italic>Chinchilla lanigera<\/jats:italic> in captivity. Currently there are no parameters to evaluate the quality of the semen of males that are sold as breeding stock, so they are selected according to their phenotypic characteristics, which cannot guarantee their reproductive efficiency. We worked with sexually mature males; the sperm were collected from the cauda epididymis by puncture. Capacitated sperm were incubated at 37 \u00b0C with different concentrations of progesterone. The results showed a biphasic behavior, where the increase in the percentage of the acrosome reaction (AR) was significant between 2.5\u20135 \u03bcM, and a maximum response with a dose of 20 \u03bcM. Also the suspension of sperm incubated with different concentrations of ionophore A23187 inducing response is obtained with a concentration of 10 \u03bcM. Stimulation of the Gamma\u2010aminobutyric acid (GABA)<jats:sub>A<\/jats:sub>\u2010like receptor by treating the sperm with different concentrations of muscimol, a specific receptor agonist, also induce acrosome reaction. The dose\u2010response curve showed a biphasic effect with a maximum response at a concentration of 2.5 \u03bcM. The pretreatment of sperm suspension with a specific receptor antagonist, picrotoxin, a decrease was observed in the percentage of reacted perm. These studies represent a first step towards the establishment of standard parameters to determine the functionality and fertilizing capacity of <jats:italic>Chinchilla<\/jats:italic> sperm. In addition, our results indicate that the <jats:italic>Chinchilla<\/jats:italic> spermatozoa can undergo the AR when treated with A23187 or progesterone at high percentages. It also shows a clear involvement of GABA<jats:sub>A<\/jats:sub>\u2010like receptors in acrosome exocytosis in sperm obtained by epididymal cauda. <jats:italic>J. Exp. Zool.317:259\u2013265, 2012<\/jats:italic>. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jez.1718","type":"journal-article","created":{"date-parts":[[2012,5,14]],"date-time":"2012-05-14T13:56:13Z","timestamp":1337003773000},"page":"259-265","source":"Crossref","is-referenced-by-count":4,"title":["Acrosome Reaction in the Epididymal Sperm of <i>Chinchilla lanigera<\/i>. Effect of Progesterone, A23187 and Stimulation of a GABA<sub>A<\/sub>\u2010Like Receptor"],"prefix":"10.1002","volume":"317","author":[{"given":"MAR\u00cdA CECILIA","family":"GRAMAJO\u2010B\u00dcHLER","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda del Desarrollo Instituto Superior de Investigaciones Biol\u00f3gicas (INSIBIO)  Chacabuco San Miguel de Tucum\u00e1n Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LILIANA","family":"ZELARAY\u00c1N","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda del Desarrollo Instituto Superior de Investigaciones Biol\u00f3gicas (INSIBIO)  Chacabuco San Miguel de Tucum\u00e1n Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ALBERTO","family":"L\u00d3PEZ LUIS","sequence":"additional","affiliation":[{"name":"Instituto de Histolog\u00eda y Embriolog\u00eda Facultad de Ciencias Medicas Universidad Nacional de Cuyo  Parque Gral. 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