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Firstly, to determine which class of receptors, glucocorticoid (GRs) or mineralocorticoid (MRs), are involved in cortisol regulation of arginine vasotocin (AVT) and isotocin (IT) release from the hypothalamo\u2010pituitary (H\u2013P) complex of round goby (<jats:italic>Neogobius melanostomus<\/jats:italic>). Secondly, to determine which pathways, genomic or non\u2010genomic, are involved in the aformentioned process.The H\u2013P explants were perfused with cortisol (1.4\u2009\u00d7\u200910<jats:sup>\u2212<\/jats:sup><jats:sup>7<\/jats:sup>\u2009M, 2.8\u2009\u00d7\u200910<jats:sup>\u22127<\/jats:sup>M, 0.4\u2009\u00d7\u200910<jats:sup>\u22126<\/jats:sup>M); only the highest dose significantly increased a release of both nonapeptides. In the perfusion of H\u2013P explants, we used cortisol (0.4\u2009\u00d7\u200910<jats:sup>\u22126<\/jats:sup>M) in combination with GRs antagonist RU486 (0.3\u2009\u00d7\u200910<jats:sup>\u22126<\/jats:sup>\u2009M) or MRs antagonist C03DA01 (0.36\u2009\u00d7\u200910<jats:sup>\u22126<\/jats:sup>\u2009M) or transcription inhibitor Actinomycin D (1\u2009\u00d7\u200910<jats:sup>\u22127<\/jats:sup>\u2009M). All inhibitors were also tested seperately. The contents of AVT and IT in the perfusion media was determined by high\u2010performance liquid chromatography (HPLC) with UV detection. This study suggested that different mechanisms were involved in the regulation of AVT and IT release from H\u2013P complex in round goby. Apparently it was GRs but not MRs that were involved in cortisol regulation of AVT and IT release. In the case of AVT, our data points to both genomic and non\u2010genomic pathways mediating the effect of cortisol; in the case of IT, it is only the non\u2010genomic pathway. This study presents the first feasible mechanisms of cortisol action on AVT and IT release from the H\u2013P complex in round goby.<jats:italic>J. Exp. Zool. 323A: 616\u2013626, 2015<\/jats:italic>. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jez.1952","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T19:16:36Z","timestamp":1436901396000},"page":"616-626","source":"Crossref","is-referenced-by-count":6,"title":["Cortisol stimulates arginine vasotocin and isotocin release from the hypothalamo\u2010pituitary complex of round goby (<i>Neogobius melanostomus<\/i>): Probable mechanisms of action"],"prefix":"10.1002","volume":"323","author":[{"given":"Hanna","family":"Kalamarz\u2010Kubiak","sequence":"first","affiliation":[{"name":"Department of Genetics and Marine Biotechnology Institute of Oceanology Polish Academy of Sciences Sopot Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Kleszczy\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Genetics and Marine Biotechnology Institute of Oceanology Polish 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