{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T18:55:31Z","timestamp":1778957731510,"version":"3.51.4"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,10,4]],"date-time":"2018-10-04T00:00:00Z","timestamp":1538611200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Exp Zool Pt A"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Catecholamines protect the fish heart during hypoxia. However, the humoral adrenergic stress response may only be invoked in extremis. We investigated the hypothesis that endogenous (e.g., neuronal) myocardial catecholamines may also impact cardiac performance during hypoxia in a hypoxia\u2010tolerant tropical fish, the red\u2010bellied piranha (\n                    <jats:italic>Pygocentrus nattereri<\/jats:italic>\n                    ). First, we measured endogenous tissue catecholamines and in vitro catecholamine release from piranha myocardium using ultraperformance liquid chromatography. Ventricle homogenates contained detectable levels of both adrenaline (7.27\u2009ng\/g) and noradrenaline (14.48\u2009ng\/g), but only noradrenaline was released from ventricular tissue incubated in Ringer\u2019s solution. Noradrenaline released in this assay was not affected by hypoxia but was promoted by the catecholamine releasing agent tyramine. Our second series of experiments explored cardiac contractile performance in vitro using tyramine, exogenous noradrenaline or adrenaline, and propranolol (a \u03b2\u2010adrenoceptor antagonist). In ventricular strip preparations, \u03b2\u2010adrenergic blockade with propranolol had no effects on twitch force or contraction kinetics in either normoxia or hypoxia, confirming that spontaneous endogenous catecholamine release did not impact cardiac performance. However, in the absence of propranolol, tyramine mimicked the positive inotropic effect of noradrenaline (10\u2009\u00b5M) during hypoxia, although adrenaline was capable of generating larger effects. Our results suggest that, although it is not spontaneously released, inducible endogenous noradrenaline release may have a significant \u03b2\u2010adrenoceptor\u2010dependent impact on hypoxic performance in the fish heart.\n                  <\/jats:p>","DOI":"10.1002\/jez.2233","type":"journal-article","created":{"date-parts":[[2018,10,5]],"date-time":"2018-10-05T23:14:29Z","timestamp":1538781269000},"page":"27-37","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The effects of endogenous and exogenous catecholamines on hypoxic cardiac performance in red\u2010bellied piranhas"],"prefix":"10.1002","volume":"331","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3782-1641","authenticated-orcid":false,"given":"William","family":"Joyce","sequence":"first","affiliation":[{"name":"Department of Zoophysiology Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine J. A.","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Zoophysiology Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofie","family":"Iversen","sequence":"additional","affiliation":[{"name":"Department of Zoophysiology Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Per Guldhammer","family":"Henriksen","sequence":"additional","affiliation":[{"name":"Department of Zoophysiology Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Bayley","sequence":"additional","affiliation":[{"name":"Department of Zoophysiology Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tobias","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Zoophysiology Aarhus University Aarhus Denmark"},{"name":"Aarhus Institute of Advanced Studies Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,4]]},"reference":[{"key":"e_1_2_7_1_2_1","doi-asserted-by":"publisher","DOI":"10.1139\/z99-019"},{"key":"e_1_2_7_1_3_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1973.sp010204"},{"key":"e_1_2_7_1_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00127.2014"},{"key":"e_1_2_7_1_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00689295"},{"key":"e_1_2_7_1_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1966.tb03403.x"},{"key":"e_1_2_7_1_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-6395.1954.tb00037.x"},{"key":"e_1_2_7_1_8_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.02781"},{"key":"e_1_2_7_1_9_1","first-page":"pp. 155","volume-title":"Fish physiology","author":"Farrell A. P.","year":"2017"},{"key":"e_1_2_7_1_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00302594"},{"key":"e_1_2_7_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(03)01180-4"},{"key":"e_1_2_7_1_12_1","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/S0022-3565(25)26363-7","article-title":"The role of myocardial catecholamines in the response to tyramine","volume":"137","author":"Gaffney T. E.","year":"1962","journal-title":"The Journal of Pharmacology and Experimental Therapeutics"},{"key":"e_1_2_7_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0010-406X(69)91627-2"},{"key":"e_1_2_7_1_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00689300"},{"key":"e_1_2_7_1_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.02237"},{"key":"e_1_2_7_1_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00360-014-0838-8"},{"key":"e_1_2_7_1_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.11.3.364"},{"key":"e_1_2_7_1_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1977.tb10353.x"},{"key":"e_1_2_7_1_19_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118916"},{"key":"e_1_2_7_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2012.08.007"},{"key":"e_1_2_7_1_21_1","doi-asserted-by":"publisher","DOI":"10.1086\/682701"},{"key":"e_1_2_7_1_22_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.130971"},{"key":"e_1_2_7_1_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1999.277.4.H1562"},{"key":"e_1_2_7_1_24_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.101.22.2645"},{"key":"e_1_2_7_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0300-9629(83)90455-3"},{"key":"e_1_2_7_1_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00359-009-0447-2"},{"key":"e_1_2_7_1_27_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007829214826"},{"key":"e_1_2_7_1_28_1","first-page":"1","article-title":"The distribution of catechol amines in lower animals and their effect on the heart","volume":"31","author":"Ostlund E.","year":"1954","journal-title":"Acta Physiologica Scandinavica Suppl"},{"key":"e_1_2_7_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0044-8486(99)00092-7"},{"key":"e_1_2_7_1_30_1","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1242\/jeb.155.1.549","article-title":"Control of catecholamine release in vivo and in situ in the Atlantic cod (Gadus morhua) during hypoxia","volume":"155","author":"Perry S. F.","year":"1991","journal-title":"Journal of Experimental Biology"},{"key":"e_1_2_7_1_31_1","doi-asserted-by":"publisher","DOI":"10.1086\/430041"},{"key":"e_1_2_7_1_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00706.2003"},{"key":"e_1_2_7_1_33_1","first-page":"285","article-title":"Plasma catecholamines in rainbow trout (Salmo gairdneri) during hypoxia","volume":"48","author":"Ristori M. T.","year":"1989","journal-title":"Experimental Biology"},{"key":"e_1_2_7_1_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autneu.2011.08.006"},{"key":"e_1_2_7_1_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00307196"},{"key":"e_1_2_7_1_36_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.55.5.689"},{"key":"e_1_2_7_1_37_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.60.2.194"},{"key":"e_1_2_7_1_38_1","doi-asserted-by":"publisher","DOI":"10.1086\/644759"},{"key":"e_1_2_7_1_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1095-6433(02)00050-8"},{"key":"e_1_2_7_1_40_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00372.2010"},{"key":"e_1_2_7_1_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23764"},{"key":"e_1_2_7_1_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0742-8413(89)90217-X"},{"key":"e_1_2_7_1_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0742-8413(86)90121-0"},{"key":"e_1_2_7_1_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-6480(61)90028-4"},{"issue":"4","key":"e_1_2_7_1_45_1","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1242\/jeb.201.4.533","article-title":"L\u2010type Ca2+ current in fish cardiac myocytes: Effects of thermal acclimation and beta\u2010adrenergic stimulation","volume":"201","author":"Vornanen M.","year":"1998","journal-title":"The Journal of Experimental Biology"},{"key":"e_1_2_7_1_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901320406"}],"container-title":["Journal of Experimental Zoology Part A: Ecological and Integrative Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjez.2233","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jez.2233","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T18:41:51Z","timestamp":1775241711000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jez.2233"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,4]]},"references-count":45,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["10.1002\/jez.2233"],"URL":"https:\/\/doi.org\/10.1002\/jez.2233","archive":["Portico"],"relation":{},"ISSN":["2471-5638","2471-5646"],"issn-type":[{"value":"2471-5638","type":"print"},{"value":"2471-5646","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,4]]},"assertion":[{"value":"2018-05-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}