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Under natural conditions, Broad\u2010snouted caiman (<jats:italic>Caiman latirostris<\/jats:italic>) eggs are influenced by thermal changes in the interior of the nest related to the external environmental temperature. As\u00a0nests are subject to variations in temperature and most lab studies on crocodilian\u00a0incubation have been\u00a0carried out at constant temperatures, we were interested in determining how temperature fluctuations may affect the development of caiman embryos. We investigated the effects of incubation at constant temperatures (31\u00b0C, 32\u00b0C, and 33\u00b0C) and fluctuating temperatures (31\u2009\u00b1\u20092, 32\u2009\u00b1\u20091, and 32\u2009\u00b1\u20092\u00b0C) on the following aspects: incubation period duration, hatching success, sex ratio, total length, and body mass of <jats:italic>C<\/jats:italic>. <jats:italic>latirostris<\/jats:italic> hatchlings. Eggs incubated at 31\u00b0C produced 100% females, those at 32\u00b0C produced 71.6% females (however, the sex ratio was nest related), and at 33\u00b0C produced 100% males. We found a masculinizing effect when incubation was at 31\u2009\u00b1\u20092\u00b0C compared with a constant 31\u00b0C; and temperature fluctuations at 32\u00b0C (32\u2009\u00b1\u20091 and 32\u2009\u00b1\u20092\u00b0C) had a negative effect on hatchlings size and mass, and hatching success compared with constant incubation temperatures of 32\u00b0C and 33\u00b0C. Finally, the effect of temperature variation during the incubation period on sex ratio, hatching success, and phenotype depends on the mean temperature, as\u00a0the fluctuation around 31\u00b0C affected the sex ratios and incubation period, and the fluctuation around 32\u00b0C affected hatchling\u00a0success and size.<\/jats:p>","DOI":"10.1002\/jez.2265","type":"journal-article","created":{"date-parts":[[2019,4,30]],"date-time":"2019-04-30T11:28:36Z","timestamp":1556623716000},"page":"299-307","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Influence of temperature variation on incubation period, hatching success, sex ratio, and phenotypes in <i>Caiman latirostris<\/i>"],"prefix":"10.1002","volume":"331","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1010-5231","authenticated-orcid":false,"given":"Melina S.","family":"Simoncini","sequence":"first","affiliation":[{"name":"CIC y TTP CONICET\u2014Prov. 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