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The present study focused on the effects of ocean acidification (OA) on key hematological parameters of the juvenile small\u2010spotted catsharks (<jats:italic>Scyliorhinus canicula<\/jats:italic>). Eggs were reared throughout the entire embryogenesis (~4 months) plus 5 additional months, in two experimental treatments (control: <jats:italic>p<\/jats:italic>CO<jats:sub>2<\/jats:sub>\u2009~\u2009400\u2009\u03bcatm; and high CO<jats:sub>2<\/jats:sub>: <jats:italic>p<\/jats:italic>CO<jats:sub>2<\/jats:sub>\u2009~\u2009900\u2009\u03bcatm, \u0394\u2009\u22120.3\u2009pH units). After blood collection, the following hematological parameters were evaluated: (a) normal blood cells count (erythrocytes, leukocytes, and thrombocytes), (b) presence of erythrocytes with nuclear abnormalities, and (c) erythrocyte nucleus to cytoplasmic ratio. Concomitantly, to determine the cardiac and hematopoietic conditions, the spleen and heart to body ratios were also assessed. The present findings indicate that the measured variables may not be affected by elevated <jats:italic>p<\/jats:italic>CO<jats:sub>2<\/jats:sub> in this temperate species, as no significant differences were observed between treatments across all the endpoints tested. Nonetheless, it is worth mentioning a decreasing trend observed in a number of thrombocytes associated with OA, which should foster further investigation, regarding other aspects of their coagulation response. Along with OA, other stressors are expected to impact marine life, such as warming and hypoxia. Thus, future research should aim to investigate the cumulative effect of these stressors on hematological parameters in sharks.<\/jats:p>","DOI":"10.1002\/jez.2333","type":"journal-article","created":{"date-parts":[[2019,12,3]],"date-time":"2019-12-03T12:15:08Z","timestamp":1575375308000},"page":"126-132","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Effects of elevated carbon dioxide on the hematological parameters of a temperate catshark"],"prefix":"10.1002","volume":"333","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0379-7505","authenticated-orcid":false,"given":"Maria Rita","family":"Pegado","sequence":"first","affiliation":[{"name":"MARE \u2010 Marine and Environmental Sciences Centre, Laborat\u00f3rio Mar\u00edtimo da Guia, Faculdade de Ci\u00eancias da Universidade de Lisboa, Avenida Nossa Senhora do Cabo  Cascais Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6526-4291","authenticated-orcid":false,"given":"Catarina P.","family":"Santos","sequence":"additional","affiliation":[{"name":"MARE \u2010 Marine and Environmental Sciences Centre, Laborat\u00f3rio Mar\u00edtimo da Guia, Faculdade de Ci\u00eancias da Universidade de Lisboa, Avenida Nossa Senhora do Cabo  Cascais Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3248-4479","authenticated-orcid":false,"given":"Marta","family":"Pimentel","sequence":"additional","affiliation":[{"name":"MARE \u2010 Marine and Environmental Sciences Centre, Laborat\u00f3rio Mar\u00edtimo da Guia, Faculdade de Ci\u00eancias da Universidade de Lisboa, Avenida Nossa Senhora do Cabo  Cascais Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2385-0775","authenticated-orcid":false,"given":"Ricardo","family":"Cyrne","sequence":"additional","affiliation":[{"name":"MARE \u2010 Marine and Environmental Sciences Centre, Laborat\u00f3rio Mar\u00edtimo da Guia, Faculdade de Ci\u00eancias da Universidade de Lisboa, Avenida Nossa Senhora do Cabo  Cascais Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Paulo","sequence":"additional","affiliation":[{"name":"MARE \u2010 Marine and Environmental Sciences Centre, Laborat\u00f3rio Mar\u00edtimo da Guia, Faculdade de Ci\u00eancias da Universidade de Lisboa, Avenida Nossa Senhora do Cabo  Cascais Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4382-1135","authenticated-orcid":false,"given":"Ana Lu\u00edsa","family":"Maulvaut","sequence":"additional","affiliation":[{"name":"MARE \u2010 Marine and Environmental Sciences Centre, Laborat\u00f3rio Mar\u00edtimo da Guia, Faculdade de Ci\u00eancias da Universidade de Lisboa, Avenida Nossa Senhora do Cabo  Cascais Portugal"},{"name":"UCIBIO, REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia Universidade Nova de Lisboa, Quinta da Torre  Caparica Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6349-7232","authenticated-orcid":false,"given":"Dayanne","family":"Raffoul","sequence":"additional","affiliation":[{"name":"MARE \u2010 Marine and Environmental Sciences Centre, Laborat\u00f3rio Mar\u00edtimo da Guia, Faculdade de Ci\u00eancias da Universidade de Lisboa, Avenida Nossa Senhora do Cabo  Cascais Portugal"},{"name":"Universit\u00e0 degli Studi di Milano\u2010Bicocca  Milano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1571-0366","authenticated-orcid":false,"given":"M\u00e1rio","family":"Diniz","sequence":"additional","affiliation":[{"name":"UCIBIO, REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia Universidade Nova de Lisboa, Quinta da Torre  Caparica Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6723-2557","authenticated-orcid":false,"given":"Regina","family":"Bispo","sequence":"additional","affiliation":[{"name":"Centro de Matem\u00e1tica e Aplica\u00e7\u00f5es &amp; 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