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To assess the toxicological effects of a xenobiotic on a nontarget organism in the laboratory, different models of toxicological exposure systems have been widely used. Therefore, the aim of this study was to evaluate and compare the action of sublethal concentrations of atrazine on the hepatic histology of <jats:italic>Oreochromis niloticus<\/jats:italic>, considering two models of exposure: static (where atrazine was only added once) and semi\u2010static (where atrazine was periodically renewed). Fish were exposed to a concentration of 2\u2009ppm atrazine for 15 days, which was verified by high\u2010performance liquid chromatography. The livers were stained with hematoxylin and eosin and histopathological data were collected. In addition, they were submitted to immunohistochemistry for inducible nitric oxide synthase (iNOS). A maximum variation of 45% (static) and 12.5% (semi\u2010static) was observed between the observed and nominal atrazine concentration. Nuclear and cytoplasmic changes were observed in both experimental models. Hepatocytes from the livers of the static system showed a degenerative appearance, while in the semi\u2010static system, intense cytoplasmic vacuolization and necrosis were observed. iNOS positive cells were identified only in macrophages in the hepatocytes of fish in the semi\u2010static system. These results directly showed how the choice of exposure system can influence the results of toxicological tests. However, future analysis investigating the by\u2010products and nitrogen products should be carried out since the histopathological findings revealed the possibility of these compounds serving as secondary contamination routes.<\/jats:p>","DOI":"10.1002\/jez.2471","type":"journal-article","created":{"date-parts":[[2021,5,5]],"date-time":"2021-05-05T14:44:12Z","timestamp":1620225852000},"page":"512-521","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Can the exposure system adopted influence the results of the atrazine toxicity in hepatic tissue of fish?"],"prefix":"10.1002","volume":"335","author":[{"given":"Anderson K. 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