{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,27]],"date-time":"2025-12-27T21:12:24Z","timestamp":1766869944813,"version":"3.40.5"},"reference-count":53,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,3,14]],"date-time":"2022-03-14T00:00:00Z","timestamp":1647216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Exp Zool Pt A"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:sc>d<\/jats:sc>\u2010galactose (DG)\u2010induced rodent aging model has widely been used for the study of age\u2010related dysfunctions of various organs, including gonads and uterus. Antidiabetic drug metformin has gained an attention as antiaging drug in model organism and human but its effect on uterus has not been studied in relation to induced aging. Therefore, we investigated the effect of metformin on uterus of DG\u2010induced aging mice model. Mice were randomly divided into three groups, that is, control (CN), DG\u2010induced aging model and aging model treated with metformin. Histomorphometric results showed significantly decreased number of uterine glands, endometrial thickness, and increased luminal epithelium height in aging model. Furthermore, metformin resumed the number of uterine glands, endometrial thickness, and luminal epithelium height up to CN group. Metformin has also significantly decreased the age\u2010associated oxidative stress (malondialdehyde and lipid hydroperoxide). Superoxide dismutase was significantly decreased in both treated groups compared to the CN group. However, catalase and glutathione peroxidase enzymes were significantly increased by metformin compared to the aging model. Immunostaining of active caspase3 and BAX were intense in the endometrium of aging model compare to CN\u2010 and metformin\u2010treated groups. Localization of B\u2010cell lymphoma 2 (Bcl2) showed intense immunostaining in the uterus of CN\u2010 and metformin\u2010treated groups, with mild immunostaining in aging model. Our observations suggested that metformin treatment might be helpful for management of age\u2010associated uterine dysfunctions. Moreover, it may be concluded that metformin might ameliorate uterine dysfunctions by reducing oxidative stress, suppressing apoptosis, and increasing the survival\/antiapoptotic protein Bcl2.<\/jats:p>","DOI":"10.1002\/jez.2592","type":"journal-article","created":{"date-parts":[[2022,3,14]],"date-time":"2022-03-14T17:59:50Z","timestamp":1647280790000},"page":"600-611","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effects of metformin on the uterus of <scp>d<\/scp>\u2010galactose\u2010induced aging mice: Histomorphometric, immunohistochemical localization (B\u2010cell lymphoma 2, Bcl2\u2010associated X protein, and active capase3), and oxidative stress study"],"prefix":"10.1002","volume":"337","author":[{"given":"Vanlal","family":"Rempuia","sequence":"first","affiliation":[{"name":"Department of Zoology Mizoram University Aizawl Mizoram India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Borgohain","family":"Anima","sequence":"additional","affiliation":[{"name":"Department of Zoology Mizoram University Aizawl Mizoram India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malsawmhriatzuala","family":"Jeremy","sequence":"additional","affiliation":[{"name":"Department of Zoology Kutir Post Graduate College, Chakkey Jaunpur India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guruswami","family":"Gurusubramanian","sequence":"additional","affiliation":[{"name":"Department of Zoology Mizoram University Aizawl Mizoram India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pranay P.","family":"Pankaj","sequence":"additional","affiliation":[{"name":"Department of Zoology Nagaland University Lumami Nagaland India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajesh K.","family":"Kharwar","sequence":"additional","affiliation":[{"name":"Department of Zoology Kutir Post Graduate College, Chakkey Jaunpur India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8973-5529","authenticated-orcid":false,"given":"Vikas K.","family":"Roy","sequence":"additional","affiliation":[{"name":"Department of Zoology Mizoram University Aizawl Mizoram India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,3,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.7.17.6625"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2018.09.010"},{"issue":"4","key":"e_1_2_9_4_1","first-page":"234","article-title":"Cardioprotective effect of metformin against doxorubicin cardiotoxicity in rats","volume":"16","author":"Argun M.","year":"2016","journal-title":"Anatolian Journal of Cardiology"},{"volume-title":"Theory and practice of histological techniques","year":"2008","author":"Bancroft J. 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