{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T15:30:12Z","timestamp":1792423812229,"version":"4.5.0"},"reference-count":54,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,11,27]],"date-time":"2022-11-27T00:00:00Z","timestamp":1669507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Exp Zool Pt A"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Lead is considered one of the most prevalent environmental and biologically hazardous toxicants among metallic elements. It severely affects human health and especially the male reproductive system by causing reproductive organ dysfunction leading to infertility. Natural dietary antioxidants are studied for their ability to ameliorate the cells'\u00a0miscellaneous damage. The current study was designed to explore the effect of <jats:italic>Vitis vinifera<\/jats:italic>\u00a0(Linn.) (grape) seed extract (GSE) on lead acetate (LA)\u2010induced oxidative damage on testis and sperm quality in rats. Twenty\u2010four male Wistar rats were allocated into four equal groups. Group I received distilled water; Group II received LA 50\u2009mg\/kg body weight (Bw); Group III received LA 50\u2009mg\/kg\u2009+\u2009GSE 200\u2009mg\/kg Bw; and Group IV received LA 50\u2009mg\/kg\u2009+\u2009GSE 400\u2009mg\/kg Bw (orally once a day for 28 days). After 28 days, levels of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx),\u00a0and reduced glutathione (GSH) were estimated in the testicular tissue. The cauda of the epididymis was used to study the characteristics of the sperm, such as\u00a0sperm count, motility, viability, tail\u2010coiled sperm, and morphology. The hematoxylin and eosin staining method was used to study histomorphology. Results revealed that LA induction significantly increased MDA concentration\u00a0and decreased the levels of SOD, CAT, GPx, and\u00a0GSH. It also reduced the weight of the testis and testosterone hormone levels, declined the quality of sperm, and increased morphologically abnormal sperm. Moreover, LA severely altered the histomorphology of the testis, such as atrophy of the seminiferous tubule, degeneration of germinal epithelium, and increased interstitial space, compared with the control group. In Groups\u00a0III and IV, coadministration of LA with GSE reduced the MDA concentration, preserved the antioxidant enzyme system and testosterone hormonal levels, restored the sperm characteristics, reduced the abnormal sperm, and improved histomorphological alterations in the testis compared with the LA\u2010induced group. In conclusion, GSE has a potent natural antioxidant that provides promising protection against LA\u2010induced testicular oxidative damage on testis and sperm quality in rats.<\/jats:p>","DOI":"10.1002\/jez.2672","type":"journal-article","created":{"date-parts":[[2022,11,28]],"date-time":"2022-11-28T05:59:55Z","timestamp":1669615195000},"page":"210-219","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Ameliorative effect of <i>Vitis vinifera<\/i> (Linn.) seed extract on lead acetate induced oxidative damage on testis and sperm quality in Wistar rats"],"prefix":"10.1002","volume":"339","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8185-7262","authenticated-orcid":false,"given":"Mahesh Babu","family":"Yallamati","sequence":"first","affiliation":[{"name":"Department of Anatomy, Sri Ramachandra Medical College Sri Ramachandra Institute of Higher Education and Research (DU)  Chennai Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5510-4614","authenticated-orcid":false,"given":"Senthil Kumar","family":"Sampath","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Sri Ramachandra Medical College Sri Ramachandra Institute of Higher Education and Research (DU)  Chennai Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasanthi","family":"Mary","sequence":"additional","affiliation":[{"name":"Centre for Toxicology and Development Research (CEFT) Sri Ramachandra Institute of Higher Education and Research (DU)  Chennai Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Zafar","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Anatomy Dr. ALM PG Institute Bio Medical Sciences  Chennai Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,11,27]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.4067\/S0718-16202011000200014"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2010.10.004"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2016.4928"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.02110s5887"},{"key":"e_1_2_11_6_1","volume-title":"Elemental speciation in human health risk assessment","author":"Apostoli P.","year":"2006"},{"key":"e_1_2_11_7_1","volume-title":"Theory and practice of histological and histochemical techniques","author":"Bancroft J. 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