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There is no consensus on which strategy is more expensive metabolically face salinity challenges: conformation with higher dependence on cell volume regulation, or hyper regulation, alleviating the need for intense cell volume regulation. Crabs were probed for their acute response to dilute seawater through exposures to salinities 35\u2030, 30\u2030, 25\u2030, and 20\u2030\u00a0for 2, 4, and 6\u2009h. Hemolymph osmolality, lactate, and ions (chloride, sodium, magnesium, potassium) were assayed, as well as muscle water content. Water dissolved oxygen, ammonia, and pH levels were also measured.<jats:italic>H. pudibundus<\/jats:italic>conformed for osmolality and displayed increase in muscle hydration along the decrease in salinity down to 25\u2030, while<jats:italic>C. danae<\/jats:italic>efficiently maintained hemolymph osmo ionic stability, consumed more oxygen, acidified more the water, and released more ammonia. In 25\u2030,\u00a0both species spent energy:<jats:italic>H. pudibundus<\/jats:italic>putatively controlling cell volume, and<jats:italic>C. danae<\/jats:italic>regulating hemolymph concentrations. In 20\u2030,<jats:italic>H. pudibundus<\/jats:italic>closed itself, avoiding the contact of the interface epithelia with the external environment and producing much lactate, whereas<jats:italic>C. danae<\/jats:italic>spent more energy (aerobic) in extracellular osmo ionic stability. Under these conditions, anisosmotic extracellular regulation (together with additional cell volume regulation) is more oxygen consuming than osmoconformation with a putatively more intense challenge to cell volume. The exposure to hyposalinity limits the occupation of estuarine environments by<jats:italic>H. pudibundus<\/jats:italic>in short and middle term.<\/jats:p>","DOI":"10.1002\/jez.2697","type":"journal-article","created":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T06:47:30Z","timestamp":1679899650000},"page":"519-534","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Acute metabolic responses of two marine brachyuran crabs to dilute seawater: The aerobic cost of hyper regulation"],"prefix":"10.1002","volume":"339","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9269-0640","authenticated-orcid":false,"given":"Leonardo P.","family":"Rios","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Zoologia Universidade Federal do Paran\u00e1 Curitiba Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3492-3893","authenticated-orcid":false,"given":"Carolina A.","family":"Freire","sequence":"additional","affiliation":[{"name":"Departamento de Fisiologia, Setor de Ci\u00eancias Biol\u00f3gicas Universidade Federal do Paran\u00e1 Curitiba Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,3,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00360-021-01354-0"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpc.2013.08.004"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/10236244.2015.1024078"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-6409.2012.00535.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps002133"},{"key":"e_1_2_9_7_1","first-page":"165","volume-title":"Osmotic and ionic regulation. 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