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This study investigates the role of the calcium\u2010activated chloride channel, Anoctamin 1 (ANO1), in the gills of the euryhaline Japanese medaka (<jats:italic>Oryzias latipes<\/jats:italic>) under hyperosmolality and cortisol (CORT) influence. Acclimation to artificial SW, NaCl, mannitol, or glucose significantly upregulated <jats:italic>ANO1<\/jats:italic> and <jats:italic>CFTR<\/jats:italic> mRNA expression in gills, unlike urea treatment. In situ hybridization revealed <jats:italic>ANO1<\/jats:italic> mRNA in chloride cells co\u2010expressing <jats:italic>CFTR<\/jats:italic> and <jats:italic>Na<\/jats:italic><jats:sup>+<\/jats:sup>, <jats:italic>K<\/jats:italic><jats:sup>+<\/jats:sup>\u2010ATPase under hyperosmotic conditions. ANO1 inhibition elevated plasma Cl<jats:sup>\u2212<\/jats:sup> concentration, indicating impaired Cl<jats:sup>\u2212<\/jats:sup> excretion. CORT or dexamethasone administration in freshwater (FW) fish significantly increased branchial <jats:italic>ANO1<\/jats:italic> and <jats:italic>CFTR<\/jats:italic> mRNA expression, an effect attenuated by the glucocorticoid receptor (GR) antagonist RU486. Hyperosmotic treatment of isolated gill tissues rapidly induced <jats:italic>ANO1<\/jats:italic> mRNA expression independent of <jats:italic>CFTR<\/jats:italic> mRNA changes, and this induction was unaffected by RU486. These findings highlight the dual regulation of ANO1 expression via hyperosmolality\u2010induced cellular response and the CORT\u2013GR system. Thus, branchial ANO1 may likely complement CFTR in Cl\u207b excretion, playing a key role in the hyperosmotic adaptation of euryhaline teleosts.<\/jats:p>","DOI":"10.1002\/jez.2894","type":"journal-article","created":{"date-parts":[[2024,12,24]],"date-time":"2024-12-24T11:38:21Z","timestamp":1735040301000},"page":"356-372","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Regulation of Branchial Anoctamin 1 Expression in Freshwater\u2010 and Seawater\u2010Acclimated Japanese Medaka, <i>Oryzias latipes<\/i>"],"prefix":"10.1002","volume":"343","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2802-6643","authenticated-orcid":false,"given":"Norifumi","family":"Konno","sequence":"first","affiliation":[{"name":"Departement of Biology, Faculty of Science, Academic Assembly University of Toyama Gofuku Toyama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayane","family":"Togashi","sequence":"additional","affiliation":[{"name":"Departement of Biology, Graduate School of Science and Engineering University of Toyama Gofuku Toyama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Miyanishi","sequence":"additional","affiliation":[{"name":"Department of Marine Biology and Environmental Sciences, Faculty of Agriculture University of Miyazaki Miyazaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morio","family":"Azuma","sequence":"additional","affiliation":[{"name":"Division of Molecular Pharmacology, Department of Pharmacology Jichi Medical University Shimotsuke Tochigi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoya","family":"Nakamachi","sequence":"additional","affiliation":[{"name":"Departement of Biology, Faculty of Science, Academic Assembly University of Toyama Gofuku Toyama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kouhei","family":"Matsuda","sequence":"additional","affiliation":[{"name":"Departement of Biology, Faculty of Science, Academic Assembly University of Toyama Gofuku Toyama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,24]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_9_2_1","DOI":"10.1016\/j.ygcen.2012.08.028"},{"doi-asserted-by":"publisher","key":"e_1_2_9_3_1","DOI":"10.1111\/j.1365-2826.2012.02282.x"},{"doi-asserted-by":"publisher","key":"e_1_2_9_4_1","DOI":"10.1006\/gcen.1994.1111"},{"doi-asserted-by":"publisher","key":"e_1_2_9_5_1","DOI":"10.1007\/s002329900372"},{"doi-asserted-by":"publisher","key":"e_1_2_9_6_1","DOI":"10.1074\/jbc.M002076200"},{"doi-asserted-by":"publisher","key":"e_1_2_9_7_1","DOI":"10.1152\/ajprenal.90228.2008"},{"doi-asserted-by":"publisher","key":"e_1_2_9_8_1","DOI":"10.1007\/s00360-016-0972-6"},{"doi-asserted-by":"publisher","key":"e_1_2_9_9_1","DOI":"10.1016\/j.cbpa.2015.04.017"},{"doi-asserted-by":"publisher","key":"e_1_2_9_10_1","DOI":"10.1007\/s00360-014-0817-0"},{"issue":"3","key":"e_1_2_9_11_1","first-page":"347","article-title":"The Presence and Role of Interstitial Cells of Cajal in the Proximal Intestine of Shorthorn Sculpin (Myoxocephalus scorpius)","volume":"220","author":"Brijs J.","year":"2017","journal-title":"Journal of Experimental Biology"},{"doi-asserted-by":"publisher","key":"e_1_2_9_12_1","DOI":"10.1152\/physrev.00056.2006"},{"doi-asserted-by":"publisher","key":"e_1_2_9_13_1","DOI":"10.1152\/ajprenal.00003.2003"},{"doi-asserted-by":"publisher","key":"e_1_2_9_14_1","DOI":"10.1016\/j.yjmcc.2013.12.027"},{"doi-asserted-by":"publisher","key":"e_1_2_9_15_1","DOI":"10.1007\/s10495-010-0505-9"},{"doi-asserted-by":"publisher","key":"e_1_2_9_16_1","DOI":"10.1242\/jeb.063057"},{"doi-asserted-by":"publisher","key":"e_1_2_9_17_1","DOI":"10.1242\/jeb.203.15.2349"},{"doi-asserted-by":"publisher","key":"e_1_2_9_18_1","DOI":"10.1016\/S0016-6480(03)00053-4"},{"key":"e_1_2_9_19_1","first-page":"58","article-title":"Effects of Cortisol Treatment and Osmotic Adaptation on the Chloride Cells in the Eel, Anguilla rostrata","volume":"6","author":"Doyle W. 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