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However, such developmental effects have been largely overlooked in other vertebrate groups. In our research, we explored the presence of developmental variation in the daily pattern of behavioral stress response in a teleost fish model: the zebrafish (<jats:italic>Danio rerio<\/jats:italic>). We compared juvenile and adult individuals in two behavioral paradigms commonly used to analyze fish stress response, such as the open\u2010field test and the diving test. Our comparisons were conducted every 4\u2009h during a 24\u2010h cycle to analyze daily variations. Significant daily rhythms were detected for almost all analyzed behaviors in both tests. In general, the analyses suggested a greater stress response in adults during the daytime and in juveniles during the night\u2010time, although not all indicators aligned in this direction. Moreover, we found average differences in zebrafish behavior, suggesting that juveniles were more sensitive to stress. 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