{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T00:00:36Z","timestamp":1771632036292,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2008,9,5]],"date-time":"2008-09-05T00:00:00Z","timestamp":1220572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Exp. Zool."],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Detecting multiple paternity in wild populations of the broad\u2010snouted caiman (<jats:italic>Caiman latirostris<\/jats:italic>) has important implications for conservation efforts. We have applied microsatellite markers to examine genetic variation in <jats:italic>C. latirostris<\/jats:italic> and also have provided the first data concerning detection of multiple paternity in wild populations of this species. Blood samples from four nest\u2010guarding <jats:italic>C. latirostris<\/jats:italic> females and their hatchlings were obtained from Santa Fe Province, Argentina. Amplified products were analyzed by electrophoresis on 10% polyacrylamide gels and visualized with silver staining. Four out of the eight markers tested reliably amplified and yielded useful data. Using polyacrylamide gels with silver staining provides high enough resolution to obtain individual genotypes. In order to assess the presence or absence of more than two parents in each nest, we used the single locus Minimum Method, and applied Cervus 3.0 and Gerud 2.0 software in parentage analyses. Our results indicate more than one father in at least two families. This behavior could be the consequence of high habitat variability in the area where our population was sampled. The ability to understand mating systems is important for maintaining viable populations of exploited taxa like <jats:italic>C. latirostris. J. Exp. Zool. 309A:637\u2013642, 2008<\/jats:italic>. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jez.496","type":"journal-article","created":{"date-parts":[[2008,9,5]],"date-time":"2008-09-05T13:39:38Z","timestamp":1220621978000},"page":"637-642","source":"Crossref","is-referenced-by-count":29,"title":["Microsatellite DNA markers applied to detection of multiple paternity in <i>Caiman latirostris<\/i> in Santa Fe, Argentina"],"prefix":"10.1002","volume":"309A","author":[{"given":"Patricia","family":"Amavet","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esteban","family":"Rosso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa","family":"Markariani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Ignacio","family":"Pi\u00f1a","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,9,5]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(91)90120-I"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0959-437X(93)90017-J"},{"key":"e_1_2_1_4_1","first-page":"38","volume-title":"Crocodilian biology and evolution","author":"Davis LM","year":"2001"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-294X.2001.01241.x"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.10189"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.2307\/1565981"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1643\/0045-8511(2002)002[1078:GDPSAG]2.0.CO;2"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2005.05.002"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.2307\/1447789"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.10206"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/jhered\/esh067"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-8286.2005.01029.x"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-294X.2003.01928.x"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1996.tb04504.x"},{"key":"e_1_2_1_16_1","first-page":"102","volume-title":"Crocodiles and alligators","author":"Lang JW","year":"1989"},{"key":"e_1_2_1_17_1","unstructured":"LarrieraA. 1992.La conservaci\u00f3n y el manejo deCaiman latirostrisen la Argentina. In: Verdade LM Lavorenti A editors. Anais do II Workshop sobre Conserva\u00e7ao e Manejo do Jacar\u00e9\u2010de\u2010Papo\u2010Amarelo (Caiman latirostris). Universidade de Sao Paulo Piracicaba: CIZBAS\/ESALQ. p8\u201317."},{"key":"e_1_2_1_18_1","unstructured":"LarrieraA. 1998.TheCaiman latirostrisranching program in Santa Fe Argentina: the first commercial rearing 1998. Crocodiles. Proceedings of the 14th Working Meeting of the Crocodile Specialist Group IUCN\u2014The World Conservation Union. Gland Switzerland and Cambridge UK. p379\u2013385."},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(88)90104-8"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-294x.1998.00374.x"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/8.19.4321"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2004.02208.x"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.101109298"},{"key":"e_1_2_1_24_1","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1093\/genetics\/137.4.1147","article-title":"Effective population size with multiple paternity","volume":"137","author":"Sugg DW","year":"1994","journal-title":"Genetics"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0959-437X(94)90067-1"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1739.1999.00011.x"},{"key":"e_1_2_1_27_1","first-page":"25","volume-title":"Memorias del IV Workshop sobre Conservaci\u00f3n y Manejo del yacar\u00e9 overo (Caiman latirostris)","author":"Tourn S","year":"1993"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.10200"},{"key":"e_1_2_1_29_1","unstructured":"VillelaPMS.2004.Caracteriza\u00e7\u00e3o gen\u00e9tica de popula\u00e7\u00f5es de jacar\u00e9\u2010de\u2010papo\u2010amarelo (Caiman latirostris) utilizando marcadores microssat\u00e9lites. 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