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The lumen negative transepithelial voltage (<jats:italic>V<\/jats:italic><jats:sub>te<\/jats:sub>) was measured and luminal alkalinization was determined through the color change of luminal m\u2010cresol purple from yellow to purple after luminal perfusion stops. Addition of 10\u2009mmol\u2009L<jats:sup>\u22121<\/jats:sup> amino acids (arginine, glutamine, histidine or proline) or dicarboxylic acids (malate or succinate) to the luminal perfusate resulted in more negative <jats:italic>V<\/jats:italic><jats:sub>te<\/jats:sub> values, whereas addition of glucose was without effect. In the presence of TRIS chloride as luminal perfusate, addition of nutrients did not change <jats:italic>V<\/jats:italic><jats:sub>te<\/jats:sub>. These results are consistent with Na<jats:sup>+<\/jats:sup>\u2010dependent absorption of amino acids and dicarboxylic acids. Effects of serotonin withdrawal indicated that nutrient absorption is stimulated by this hormone. Strong luminal alkalinization was observed with mosquito saline containing serotonin on the hemolymph\u2010side and 100\u2009mmol\u2009L<jats:sup>\u22121<\/jats:sup> NaCl in the lumen, indicating that alkalinization does not depend on luminal nutrients. Omission of glucose or dicarboxylic acids from the hemolymph\u2010side solution had no effect on luminal alkalinization, whereas omission of amino acids significantly decelerated it. Re\u2010addition of amino acids restored alkalinization, suggesting the involvement of amino acid metabolism in luminal alkalinization. <jats:italic>J. Exp. 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