{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:44:48Z","timestamp":1791049488369,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,3,30]],"date-time":"2001-03-30T00:00:00Z","timestamp":985910400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Exp. Zool."],"published-print":{"date-parts":[[2001,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Since the discovery of the sex\u2010determining gene, <jats:italic>Sry<\/jats:italic>, a number of genes have been identified which are involved in sex determination and gonadogenesis in mammals. Although <jats:italic>Sry<\/jats:italic> is known to be the testis\u2010determining factor in mammals, this is not the case in non\u2010mammalian vertebrates. <jats:italic>Sox9<\/jats:italic> is another gene that has been shown to have a male\u2010specific role in sex determination, but, unlike <jats:italic>Sry, Sox9<\/jats:italic> has been shown to be involved in sex determination in mammals, birds, and reptiles. This is the first gene to be described that has a conserved role in sex determination in species with either chromosomal or environmental sex\u2010determining mechanisms. Many reptiles do not have sex chromosomes but exhibit temperature\u2010dependent sex determination (TSD). <jats:italic>Sox9<\/jats:italic> has been shown to be expressed in both turtle and alligator during gonadogenesis. To determine if <jats:italic>Sox9<\/jats:italic> also has a role in a gecko species with TSD, we studied gonadal expression of <jats:italic>Sox9<\/jats:italic> during embryonic development of the Leopard gecko (<jats:italic>Eublepharis macularius<\/jats:italic>). Gecko <jats:italic>Sox9<\/jats:italic> was found to be highly conserved at the nucleotide level when compared to other vertebrate species including human, chick, alligator, and turtle. <jats:italic>Sox9<\/jats:italic> was found to be expressed in embryos incubated at the male\u2010producing temperature (32.5\u00b0C) as well as in embryos incubated at the female\u2010producing temperatures (26 and 34\u00b0C), Northern blot analysis showed that <jats:italic>Sox9<\/jats:italic> was expressed at both temperatures from morphological stages 31 to 37. mRNA in situ hybridisation on isolated urogenital systems showed expression at both female\u2010 and male\u2010producing temperatures up to stage 36. After this stage, no expression was seen in the female gonads but expression remained in the male. These data provide further evidence that <jats:italic>Sox9<\/jats:italic> is an essential component of a testis\u2010determining pathway that is conserved in species with differing sex\u2010determining mechanisms. <jats:italic>J. Exp. Zool. (Mol. Dev. Evol.) 291:85\u201391, 2001<\/jats:italic>. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jez.7","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T20:13:04Z","timestamp":1030306384000},"page":"85-91","source":"Crossref","is-referenced-by-count":28,"title":["Characterisation and expression of <i>Sox9<\/i> in the Leopard gecko, <i>Eublepharis macularius<\/i>"],"prefix":"10.1002","volume":"291","author":[{"given":"Elizabeth M.A.","family":"Valleley","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth J.","family":"Cartwright","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nirvana J.","family":"Croft","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander F.","family":"Markham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. Louise","family":"Coletta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,12,26]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402410118"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.2.3.218"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/372525a0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/346245a0"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"2521","DOI":"10.1242\/dev.125.13.2521","article-title":"Involvement of Sox1, 2 and 3 in the early and subsequent molecular events of lens induction","volume":"125","author":"Kamachi Y","year":"1998","journal-title":"Development"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.9.2813"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/351117a0"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/21.10.2493"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.34.24023"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0996-62"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1996.8487"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/26.2.469"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.6.1473"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-010X(19980801)281:5<417::AID-JEZ7>3.0.CO;2-R"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0696-230"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1090.1979.tb00531.x"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0925-4773(94)00299-3"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402650610"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90041-8"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(199903)214:3<171::AID-AJA1>3.0.CO;2-S"},{"key":"e_1_2_1_22_1","first-page":"75","volume-title":"In situ hybridisation. A practical approach","author":"Wilkinson DG","year":"1992"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/21.3.744"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0195-15"}],"container-title":["Journal of Experimental Zoology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjez.7","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jez.7","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T13:37:57Z","timestamp":1700314677000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jez.7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,4,15]]},"references-count":23,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2001,4,15]]}},"alternative-id":["10.1002\/jez.7"],"URL":"https:\/\/doi.org\/10.1002\/jez.7","archive":["Portico"],"relation":{},"ISSN":["0022-104X","1097-010X"],"issn-type":[{"value":"0022-104X","type":"print"},{"value":"1097-010X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,4,15]]}}}