{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T11:27:19Z","timestamp":1745839639697,"version":"3.38.0"},"reference-count":41,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2011,8,12]],"date-time":"2011-08-12T00:00:00Z","timestamp":1313107200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Exp. Zool."],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Melatonin, nocturnally secreted by the pineal gland, regulates a variety of physiological functions, including reproduction. Here, we investigated the evidence of melatonin binding sites in frog tissue (brain, retina, and testis) through saturation and competition binding experiments. In the frog,<jats:italic>Pelophylax esculentus<\/jats:italic>, our results confirm the presence of a single class of melatonin\u2010specific binding sites in the brain and retina, but not in the testis. Further experiments have been done using biomolecular approaches (PCR analysis). Here, we report the isolation of a cDNA encoding for a melatonin receptor type (mel1b) from brain, retina, and testis of the<jats:italic>P. esculentus<\/jats:italic>. PCR analysis revealed that melatonin expression is higher in the brain and retina, whereas it is lower in the testis. The presence of a melatonin receptor transcript in the frog testis corroborates our previous results obtained in in vitro experiments that suggest that melatonin might act directly in male vertebrate gonads, and indicates that the frog testis may be a suitable model to verify the role of indolamine in testicular activity.<jats:italic>J. Exp. Zool. 315:520\u2013526, 2011<\/jats:italic>. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jez.700","type":"journal-article","created":{"date-parts":[[2011,8,19]],"date-time":"2011-08-19T10:07:27Z","timestamp":1313748447000},"page":"520-526","source":"Crossref","is-referenced-by-count":2,"title":["First evidence of a cDNA encoding for a melatonin receptor (mel 1b) in brain, retina, and testis of<i>Pelophylax esculentus<\/i>"],"prefix":"10.1002","volume":"315A","author":[{"given":"Ismene","family":"Serino","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaia","family":"Izzo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana","family":"Ferrara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Minucci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michela","family":"d'Istria","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,8,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/2.3.225"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.162.3850.277"},{"key":"e_1_2_5_4_1","first-page":"266","article-title":"Role of the pineal gland and melatonin in seasonal reproductive function in mammals","volume":"8","author":"Arendt J","year":"1986","journal-title":"Oxf Rev Reprod Biol"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1330005"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.1994.tb00112.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.1993.tb00903.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000124106"},{"key":"e_1_2_5_9_1","doi-asserted-by":"crossref","first-page":"1536","DOI":"10.1016\/S0022-3565(25)39545-5","article-title":"Age\u2010related changes in melatonin modulation of sympathetic neurotransmission","volume":"266","author":"Carneiro RC","year":"1993","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_5_10_1","first-page":"S73","article-title":"Melatonin, the pineal gland and circadian rhythms","volume":"8","author":"Cassone VM","year":"1993","journal-title":"J Biol Rhythms"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(01)01097-9"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.142.7.3231"},{"key":"e_1_2_5_13_1","unstructured":"De RienzoG AnielloF FerraraD MinucciS SerinoI d'IstriaM. 2001b.Expression of MT1 and melatonin related receptor (H9) in adult rat testis. In: Monduzzi editor. Perspective in Comparative Endocrinology: Unity and Diversity. International Proceedings Division Sorrento (Napoli) Italy May 26\u201330 2001. p1075\u20131079."},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1260083"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199404002898"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.2.12.2842214"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2004-0990"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/074873001129002042"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2003.10.003"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2004.00190.x"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.00786"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199409990293"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjp.2008.184"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(95)12183-8"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1159\/000109490"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02245542"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(91)90087-9"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1949.tb27297.x"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(96)00434-1"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1159\/000014637"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.3109\/09513590903159649"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(90)90020-R"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.136.12.5357"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1530\/eje.0.1360633"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0230299"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(98)00530-8"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-128X(97)81440-7"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/0-306-46814-X_22"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(88)80599-4"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(93)90173-D"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1159\/000113622"}],"container-title":["Journal of Experimental Zoology Part A: Ecological Genetics and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjez.700","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jez.700","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,8]],"date-time":"2025-03-08T23:14:27Z","timestamp":1741475667000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jez.700"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,8,12]]},"references-count":41,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2011,11]]}},"alternative-id":["10.1002\/jez.700"],"URL":"https:\/\/doi.org\/10.1002\/jez.700","archive":["Portico"],"relation":{},"ISSN":["1932-5223","1932-5231"],"issn-type":[{"type":"print","value":"1932-5223"},{"type":"electronic","value":"1932-5231"}],"subject":[],"published":{"date-parts":[[2011,8,12]]}}}