{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T03:27:07Z","timestamp":1782962827364,"version":"3.54.5"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:00:00Z","timestamp":1758672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Exp Zool Pt A"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The serine\/threonine phosphatase PP1\u03b32 plays a critical role in modulating epididymal sperm maturation and motility. Our previous work demonstrated that PP1\u03b32 regulates these processes in tree shrews via phosphorylation and dephosphorylation. This study further investigates the interactions among PP1\u03b32, SRC tyrosine kinase, and protein kinase A (PKA), along with their molecular mechanisms in regulating epididymal sperm motility in tree shrews.Using Western blotting, immunokinase assays, co\u2010immunoprecipitation, and Ser\/Thr phosphatase activity assays, we characterized key signaling dynamics. Results showed that Ser\/Thr phosphatase activity was significantly higher in caput than in cauda epididymal spermatozoa (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05). Inhibiting PKA and SRC significantly increased Ser\/Thr phosphatase activity and reduced cauda sperm motility (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05). Conversely, SRC activation with sc\u20103052 significantly reduced phosphatase activity in caput sperm (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05). We also found that SRC interacts with PP1\u03b32 in caudal sperm, and that both SRC and PKA activities were higher in caudal than in caput sperm, with SRC acting downstream of PKA. These results suggest that SRC kinase regulates sperm motility by inhibiting PP1\u03b32\u2010mediated Ser\/Thr phosphatase activity, under the regulatory control of PKA. This study provides a foundation for further investigation into PP1\u03b32's functions and reproductive regulatory mechanisms in tree shrew sperm.\n                  <\/jats:p>","DOI":"10.1002\/jez.70039","type":"journal-article","created":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T11:21:25Z","timestamp":1758712885000},"page":"27-38","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Inhibition of PP1\u03b32 by PKA Through SRC Initiates Sperm Motility in Tree Shrews"],"prefix":"10.1002","volume":"345","author":[{"given":"Minghua","family":"Yang","sequence":"first","affiliation":[{"name":"Faculty of Animal Science and Technology Yunnan Agricultural University Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Technology Yunnan Agricultural University Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Tan","sequence":"additional","affiliation":[{"name":"Shenzhen Dakewe Bio\u2010engineering Co. Ltd Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yahui","family":"Li","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Technology Yunnan Agricultural University Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.03055"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2020.110992"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2010.04.028"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gat033"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/clinem\/dgaa937"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080360211"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)37144-2"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2022.08.018"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2019.00341"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.08.002"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2416"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgs021"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092160407"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.20170"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2202-10-74"},{"issue":"2","key":"e_1_2_10_17_1","first-page":"59","article-title":"Development of Application of Tree Shrew in Human Disease Animal Models Research","volume":"30","author":"Huang X. 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