{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T10:03:56Z","timestamp":1781172236668,"version":"3.54.1"},"reference-count":53,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T00:00:00Z","timestamp":1778371200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T00:00:00Z","timestamp":1778371200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Exp Zool Pt A"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Microplastics (MPs) are tiny particles with a diameter of less than 5\u2009mm generated from the breakdown of plastic waste. In recent years, MPs have extensively entered aquatic environments and accumulated in fish tissues, including the intestine, liver, kidney, and muscle. Previous studies have demonstrated that MP exposure can damage fish organs such as the gastrointestinal tract, gills, and liver, and may pose potential health risks to humans through the food chain. However, the toxicological effects of long\u2010term MP exposure on the muscle tissue of grass carp (\n                    <jats:italic>Ctenopharyngodon idella<\/jats:italic>\n                    ), an important edible fish species, remain poorly understood. In this study, we employed an integrated transcriptomic and metabolomic approach to investigate the molecular pathways and metabolic processes in grass carp muscle under 30\u2010week long\u2010term MP exposure. At the transcriptomic level, a total of 1549 genes were upregulated, whereas 1777 genes were downregulated. Gene Ontology (GO) enrichment analysis revealed that 66.6% of differentially expressed genes (DEGs) were assigned to cellular components (CC), with the extracellular space and extracellular region being the most significantly enriched terms. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that ECM\u2010receptor interaction, focal adhesion, and PI3K\u2010Akt signaling pathways were significantly affected. At the metabolomic level, 118 metabolites were significantly upregulated, whereas 132 metabolites were significantly downregulated, with heterocyclic compounds, alcohols, and amines being notably decreased. KEGG enrichment analysis revealed that these differential metabolites were primarily involved in ABC transporters, sphingolipid metabolism, and\n                    <jats:sc>d<\/jats:sc>\n                    \u2010amino acid metabolism pathways. Integrated analysis suggested that long\u2010term MP exposure induces ECM remodeling and membrane lipid metabolism abnormalities by modulating ECM\u2010related genes and signaling pathways, and may compromise fish muscle quality through mechanisms involving oxidative stress and inflammatory responses. Overall, this study provides novel evidence for the toxicological effects and molecular mechanisms underlying long\u2010term MP exposure and offers important insights for sustainable aquaculture practices and food safety risk assessment.\n                  <\/jats:p>","DOI":"10.1002\/jez.70095","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T05:02:52Z","timestamp":1778475772000},"page":"619-631","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Thirty Weeks of Microplastics Exposure Alters Muscle Quality in Grass Carp (\n                    <i>Ctenopharyngodon idella<\/i>\n                    ) Revealed by Transcriptomics and Metabolomics"],"prefix":"10.1002","volume":"345","author":[{"given":"Minmin","family":"Xie","sequence":"first","affiliation":[{"name":"College of Life Sciences Sichuan Normal University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Xiang","sequence":"additional","affiliation":[{"name":"Chengdu Academy of Environmental Sciences Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingpeng","family":"Zheng","sequence":"additional","affiliation":[{"name":"College of Life Sciences Sichuan Normal University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixuan","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Life Sciences Sichuan Normal University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pinpin","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Sciences Sichuan Normal University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Annaroad Gene Technology Co. 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Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River Neijiang Normal University Neijiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0976-1215","authenticated-orcid":false,"given":"Chuang","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Life Sciences Sichuan Normal University Chengdu China"},{"name":"Key Laboratory of Bio\u2010Resources and Eco\u2010Environment of Ministry of Education, College of Life Sciences Sichuan University Chengdu China"},{"name":"Observation and Research Station of Sichuan Province of Fish Resources and Environment in Upper Reaches of the Yangtze River, College of Life Sciences Sichuan University Chengdu 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