{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:44:22Z","timestamp":1788871462507,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,1,20]],"date-time":"2005-01-20T00:00:00Z","timestamp":1106179200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004240","name":"Academy of Sciences of the Czech Republic","doi-asserted-by":"crossref","award":["IAA 6011201"],"award-info":[{"award-number":["IAA 6011201"]}],"id":[{"id":"10.13039\/501100004240","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004240","name":"Academy of Sciences of the Czech Republic","doi-asserted-by":"crossref","award":["AVOZ 50 11922"],"award-info":[{"award-number":["AVOZ 50 11922"]}],"id":[{"id":"10.13039\/501100004240","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007397","name":"Charles University","doi-asserted-by":"crossref","award":["216\/2004"],"award-info":[{"award-number":["216\/2004"]}],"id":[{"id":"10.13039\/100007397","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Exp. Zool."],"published-print":{"date-parts":[[2005,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The role of aldosterone in regulation of electrogenic Na\n                    <jats:sup>+<\/jats:sup>\n                    transport is well established, though mineralocorticoid receptors bind glucocorticoids with similar binding affinity as aldosterone and plasma concentration of aldosterone is much lower than glucocorticoids. In mammals, the aldosterone specificity is conferred on the low\u2010selective mineralocorticoid receptors by glucocorticoid inactivating enzyme 11\u03b2\u2010hydroxysteroid dehydrogenase (11HSD) that converts cortisol or corticosterone into metabolites (cortisone, 11\u2013dehydrocorticosterone) with lower affinity for these receptors. The present study examined the chicken intestine, whether changes in 11HSD activity are able to modulate the effect of corticosterone on Na\n                    <jats:sup>+<\/jats:sup>\n                    transport, and how the metabolism of this hormone is distributed within the intestinal wall. This study shows that not only aldosterone, but also corticosterone (B), was able to increase the electrogenic Na\n                    <jats:sup>+<\/jats:sup>\n                    transport in chicken caecum in vitro. The effect of corticosterone was higher in the presence of carbenoxolone, an inhibitor of steroid dehydrogenases, and was comparable to the effect of aldosterone. The metabolism of B in the intestine was studied; results showed oxidation of this steroid to 11\u2013dehydrocorticosterone (A) and reduction to 11\u2013dehydro\u201320\u03b2\u2013dihydrocorticosterone (20diA) as the main metabolic products at low nanomolar concentration of the substrate. In contrast, 20\u03b2\u2013dihydrocorticosterone and 20diA were the major products at micromolar concentration of B. Progesterone was converted to 20\u03b2\u2010dihydroprogesterone. The metabolism of corticosterone was localized predominantly in the intestinal mucosa (enterocytes). In conclusion, the oxidation at position C\n                    <jats:sub>11<\/jats:sub>\n                    and reduction at position C\n                    <jats:sub>20<\/jats:sub>\n                    suggest that both 11HSD and 20\u03b2\u2010hydroxysteroid dehydrogenase (20HSD) operate in the chicken intestine and that the mucosa of avian intestine possesses a partly different system of modulation of corticosteroid signals than mammals. This system seems to protect the aldosterone target tissue against excessive concentration of corticosterone and progesterone.\n                    <jats:italic>J. Exp. Zool. 303A:113\u2013122, 2005<\/jats:italic>\n                    . \u00a9 2005 Wiley\u2010Liss, Inc.\n                  <\/jats:p>","DOI":"10.1002\/jez.a.132","type":"journal-article","created":{"date-parts":[[2005,1,20]],"date-time":"2005-01-20T13:48:38Z","timestamp":1106228918000},"page":"113-122","source":"Crossref","is-referenced-by-count":16,"title":["Glucocorticoid metabolism and Na\n                    <sup>+<\/sup>\n                    transport in chicken 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