{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T23:06:23Z","timestamp":1778540783330,"version":"3.51.4"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,2,21]],"date-time":"2006-02-21T00:00:00Z","timestamp":1140480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Exp. Zool."],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Paired Y\u2010organs secrete ecdysteroid hormones that control cycles of growth and molting in crustaceans. Y\u2010Organs are regulated, at least in part, by molt\u2010inhibiting hormone (MIH), a polypeptide produced and released by the X\u2010organ\/sinus gland complex of the eyestalks. In the present studies, crab (<jats:italic>Callinectes sapidus<\/jats:italic>) Y\u2010organs were incubated in vitro in the presence of [<jats:sup>35<\/jats:sup>S]methionine, and cyclic nucleotide analogs or experimental agents that influence the cAMP signaling pathway. In 4\u2010hr incubations, 8\u2010Br\u2010cAMP and db\u2010cAMP (but not 8\u2010Br\u2010cGMP) suppressed incorporation of [<jats:sup>35<\/jats:sup>S]methionine into Y\u2010organ proteins; the effect of 8\u2010Br\u2010cAMP was concentration\u2010dependent. Autoradiograms of radiolabeled Y\u2010organ proteins separated on SDS\u2010PAGE gels indicated the effect of 8\u2010Br\u2010cAMP was general (as opposed to selective) suppression of protein synthesis. Addition of both forskolin (an adenylyl cyclase activator) and 3\u2010isobutyl\u20101\u2010methylxanthine (a phosphodiesterase inhibitor) likewise suppressed incorporation of [<jats:sup>35<\/jats:sup>S]methionine into Y\u2010organ proteins. Cycloheximide (a protein synthesis inhibitor) suppressed incorporation of [<jats:sup>35<\/jats:sup>S]methionine into Y\u2010organ proteins and secretion of ecdysteroids. The combined results suggest that cAMP is involved in regulation of protein synthesis in <jats:italic>C. sapidus<\/jats:italic> Y\u2010organs. We are currently investigating the link of protein synthesis to ecdysteroid production, and the possibility of cross\u2010talk between cAMP and other cellular signaling pathways in Y\u2010organs. <jats:italic>J. Exp. 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