{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:34:27Z","timestamp":1791120867385,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,10,10]],"date-time":"2005-10-10T00:00:00Z","timestamp":1128902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Exp Zool Pt B"],"published-print":{"date-parts":[[2006,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ameloblastin is an enamel\u2010specific protein that plays critical roles in enamel formation, as well as adhesion between ameloblasts and the enamel matrix, as shown by analyses of ameloblastin\u2010null mice. In the present study, we produced two distinct antibodies that recognize the N\u2010terminus and C\u2010terminus regions of caiman ameloblastin, in order to elucidate the fate of ameloblastin peptides during tooth development. An immunohistochemical study using the antibodies showed that caiman ameloblastin was a tooth\u2010specific matrix protein that may initially be cleaved into two groups, N\u2010 and C\u2010terminal peptides, as shown in mammals. The distribution of the N\u2010terminal peptides was much different from that of the C\u2010terminal peptides during enamel formation; however, it was similar to that of mammalian ameloblastin. Although ameloblastin is thought to have a relationship with the enamel prismatic structure in mammals, in the caiman, which has non\u2010prismatic enamel, functional ameloblastin has no relationship with any enamel structure. Consequently, it is suggested that ameloblastin has kept its original functions during the evolutionary transition from reptiles to mammals and that it has been conserved in both lineages during more than 200 million years of evolution. Our results support the notion that ameloblastin acts as a factor for ameloblast adhesion to enamel matrix, because distribution of the C\u2010terminal peptides was consistently restricted on the surface layers of enamel matrix specimens ranging from immature to nearly completely mature. The principal molecules that provide the adhesive function are presumably C\u2010terminal peptides.<jats:italic>J. Exp. Zool. (Mol. Dev. Evol.) 306B, 2006<\/jats:italic>. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jez.b.21077","type":"journal-article","created":{"date-parts":[[2005,10,10]],"date-time":"2005-10-10T21:48:01Z","timestamp":1128980881000},"page":"126-133","source":"Crossref","is-referenced-by-count":8,"title":["Expression of ameloblastin during enamel formation in a crocodile"],"prefix":"10.1002","volume":"306B","author":[{"given":"Seikou","family":"Shintani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuhiko","family":"Kobata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Toyosawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Ooshima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,10,10]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0909-8836.1998.eos106510.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650110703"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.1999.4130"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650110704"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200409077"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345970760020501"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.8.4431"},{"key":"e_1_2_1_9_1","first-page":"1141","article-title":"Ameloblastin expression in rat incisors and human tooth germs","volume":"40","author":"Lee SK","year":"1996","journal-title":"Int J Dev Biol"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/S1016-8478(23)13730-7","article-title":"The structure of the rat ameloblastin gene and its expression in amelogenesis","volume":"15","author":"Lee SK","year":"2003","journal-title":"Mol Cells"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004180050136"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215549804600806"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345980770030901"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(01)00153-6"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M300445200"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.08.207"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.1998.tb02188.x"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.10148"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(01)00848-4"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(03)00767-4"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.3109\/03008209809023907"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(00)00379-6"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215549704501002"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.1998.tb02191.x"}],"container-title":["Journal of Experimental Zoology Part B: Molecular and Developmental Evolution"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjez.b.21077","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jez.b.21077","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,30]],"date-time":"2024-01-30T21:42:57Z","timestamp":1706650977000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jez.b.21077"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,10,10]]},"references-count":23,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2006,3,15]]}},"alternative-id":["10.1002\/jez.b.21077"],"URL":"https:\/\/doi.org\/10.1002\/jez.b.21077","archive":["Portico"],"relation":{},"ISSN":["1552-5007","1552-5015"],"issn-type":[{"value":"1552-5007","type":"print"},{"value":"1552-5015","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,10,10]]}}}