{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T11:31:22Z","timestamp":1792150282719,"version":"4.4.0"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,4,13]],"date-time":"2006-04-13T00:00:00Z","timestamp":1144886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Exp Zool Pt B"],"published-print":{"date-parts":[[2006,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Scales of lizards contain beta\u2010keratin of poorly known composition. In the present study, a rat polyclonal serum against a lizard beta\u2010keratin of 14\u201315\u2009kDa has been produced and the relative protein has been immunolocalized in the epidermis. The observations for the first time show that the isolated protein band derives from the extraction of a protein component of the beta\u2010keratin filaments of lizard epidermis. In immunoblots and immunocytochemistry, the antiserum recognizes most lizard beta\u2010keratins, but produces a variable cross\u2010reactivity with snake beta\u2010keratins, and weak or no reactivity with beta\u2010keratins isolated from tuatara, turtles, alligator and birds. In bidimensional immunoblots of lizard epidermis, three main spots at 15\u201316\u2009kDa with isoelectric point at 7.0, 7.6 and 8.0, and an unresolved large spot at 29\u201330\u2009kDa and with p<jats:italic>I<\/jats:italic> at 7.5\u20138.0, are obtained, may be derived from the aggregation of smaller beta\u2010keratin proteins. The ultrastructural immunolocalization with the antibody against lizard beta\u2010keratin shows that only small and large beta\u2010keratin filaments of beta\u2010cells of lizard epidermis are labeled. Keratin bundles in oberhautchen cells are less immunolabeled. Beta\u2010keratin is rapidly polymerized into beta\u2010packets that merge into larger beta\u2010keratin filaments. No labeling is present over other cell organelles or cell layers of lizard epidermis, and is absent in non\u2010epidermal cells. The antiserum recognizes epitope(s) characteristics for lizard beta\u2010keratins, partially recognized in snakes and absent in non\u2010lepidosaurian species. This result indicates that beta\u2010keratins among different reptilian groups posses different immunoreactive regions. <jats:italic>J. Exp. Zool. (Mol. Dev. Evol.) 306B, 2006<\/jats:italic>. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jez.b.21105","type":"journal-article","created":{"date-parts":[[2006,4,13]],"date-time":"2006-04-13T20:49:50Z","timestamp":1144961390000},"page":"528-538","source":"Crossref","is-referenced-by-count":20,"title":["Immunological characterization and fine localization of a lizard beta\u2010keratin"],"prefix":"10.1002","volume":"306B","author":[{"given":"Lorenzo","family":"Alibardi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mattia","family":"Toni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,4,13]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1054\/tice.2000.0099"},{"key":"e_1_2_1_3_1","first-page":"71","article-title":"Immunoreactivity of alpha\u2010 and beta\u2010layers in lizard epidermis","volume":"132","author":"Alibardi L","year":"2002","journal-title":"Belg J Zool"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.10181"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.10145"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000083732"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.a.20153"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4687(200012)246:3<179::AID-JMOR2>3.0.CO;2-D"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1022"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2004.06.001"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2004.06.002"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1401740211"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1401870212"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402430310"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20581"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0141-8130(96)01129-4"},{"key":"e_1_2_1_17_1","unstructured":"FraserRD MacraeTP RogersGE.1972. 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