{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:14:59Z","timestamp":1790864099130,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,12,19]],"date-time":"2008-12-19T00:00:00Z","timestamp":1229644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Exp Zool Pt B"],"published-print":{"date-parts":[[2009,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The mouse incisor has two unusual features: it grows continuously and it is covered by enamel exclusively on the labial side. The continuous growth is driven in part by epithelial stem cells in the cervical loop region that can both self\u2010renew and give rise to ameloblasts. We have previously reported that ectopic enamel is found on the lingual side of the incisor in mice with loss\u2010of\u2010function of sprouty (spry) genes. <jats:italic>Spry2<\/jats:italic><jats:sup>+\/\u2212<\/jats:sup>; <jats:italic>Spry4<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice, in which three sprouty alleles have been inactivated, have ectopic enamel as a result of upregulation of epithelial\u2010mesenchymal FGF signaling in the lingual part of the cervical loop. Interestingly, lingual enamel is also present in the early postnatal period in <jats:italic>Spry4<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice, in which only two sprouty alleles have been inactivated, but ectopic enamel is not found in adults of this genotype. To explore the mechanisms underlying the disappearance of lingual enamel in <jats:italic>Spry4<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> adults, we studied the fate of the lingual enamel in <jats:italic>Spry4<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice by comparing the morphology and growth of their lower incisors with wild type and <jats:italic>Spry2<\/jats:italic><jats:sup>+\/\u2212<\/jats:sup>; <jats:italic>Spry4<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice at several timepoints between the perinatal period and adulthood. Ameloblasts and enamel were detected on the lingual side in postnatal <jats:italic>Spry2<\/jats:italic><jats:sup>+\/\u2212<\/jats:sup>; <jats:italic>Spry4<\/jats:italic><jats:sup>+\/\u2212<\/jats:sup> incisors. By contrast, new ectopic ameloblasts ceased to differentiate after postnatal day 3 in <jats:italic>Spry4<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> incisors, which was followed by a progressive loss of lingual enamel. Both the posterior extent of lingual enamel and the time of its last deposition were variable early postnatally in <jats:italic>Spry4<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> incisors, but in all <jats:italic>Spry4<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> adult incisors the lingual enamel was ultimately lost through continuous growth and abrasion of the incisor. <jats:italic>J. Exp. Zool. (Mol. Dev. Evol.) 312B:473\u2013485, 2009<\/jats:italic>. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jez.b.21254","type":"journal-article","created":{"date-parts":[[2008,12,25]],"date-time":"2008-12-25T11:51:15Z","timestamp":1230205875000},"page":"473-485","source":"Crossref","is-referenced-by-count":20,"title":["Temporal analysis of ectopic enamel production in incisors from sprouty mutant mice"],"prefix":"10.1002","volume":"312B","author":[{"given":"Tomas","family":"Boran","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata","family":"Peterkova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herve","family":"Lesot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David B.","family":"Lyons","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miroslav","family":"Peterka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ophir D.","family":"Klein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,12,19]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.1989.tb00732.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345670460021201"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0909-8836.1999.eos107611.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80919-8"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.147.1.105"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1242\/dev.129.6.1533","article-title":"FGF10 maintains stem cell compartment in developing mouse incisors","volume":"129","author":"Harada H","year":"2002","journal-title":"Development"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.1985.tb00311.x"},{"key":"e_1_2_1_9_1","first-page":"531","article-title":"Morphogenesis of the lower incisor in the mouse from the bud to early bell stage","volume":"43","author":"Kieffer S","year":"1999","journal-title":"Int J Dev Biol"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.015081"},{"key":"e_1_2_1_11_1","first-page":"1017","article-title":"Mouse molar morphogenesis revisited by three\u2010dimensional reconstruction. 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