{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T01:56:49Z","timestamp":1791079009516,"version":"4.1.0"},"reference-count":73,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,4,19]],"date-time":"2013-04-19T00:00:00Z","timestamp":1366329600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Grant Agency of the Czech Republic","doi-asserted-by":"crossref","award":["CZ:GA \u010cR:GAP305\/12\/1766"],"award-info":[{"award-number":["CZ:GA \u010cR:GAP305\/12\/1766"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Exp Zool Pt B"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>In mice, a toothless diastema separates the single incisor from the three molars in each dental quadrant. In the prospective diastema of the embryo, small rudimentary buds are found that are presumed to be rudiments of suppressed teeth. A supernumerary tooth occurs in the diastema of adult mice carrying mutations in either <jats:italic>Spry2<\/jats:italic> or <jats:italic>Spry4<\/jats:italic>. In the case of <jats:italic>Spry2<\/jats:italic> mutants, the origin of the supernumerary tooth involves the revitalization of a rudimentary tooth bud (called R2), whereas its origin in the <jats:italic>Spry4<\/jats:italic> mutants is not known. In addition to R2, another rudimentary primordium (called MS) arises more anteriorly in the prospective diastema. We investigated the participation of both rudiments (MS and R2) in supernumerary tooth development in <jats:italic>Spry2<\/jats:italic> and <jats:italic>Spry4<\/jats:italic> mutants by comparing morphogenesis, proliferation, apoptosis, size and <jats:italic>Shh<\/jats:italic> expression in the dental epithelium of MS and R2 rudiments. Increased proliferation and decreased apoptosis were found in MS and R2 at embryonic day (ED) 12.5 and 13.5 in <jats:italic>Spry2<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> embryos. Apoptosis was also decreased in both rudiments in <jats:italic>Spry4<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> embryos, but the proliferation was lower (similar to WT mice), and supernumerary tooth development was accelerated, exhibiting a cap stage by ED13.5. Compared to <jats:italic>Spry2<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> mice, a high number of <jats:italic>Spry4<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> supernumerary tooth primordia degenerated after ED13.5, resulting in a low percentage of supernumerary teeth in adults. We propose that Sprouty genes were implicated during evolution in reduction of the cheek teeth in Muridae, and their deletion can reveal ancestral stages of murine dental evolution. J. Exp. Zool. (Mol. Dev. Evol.) 320B:307\u2013320, 2013. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jez.b.22502","type":"journal-article","created":{"date-parts":[[2013,4,19]],"date-time":"2013-04-19T15:30:46Z","timestamp":1366385446000},"page":"307-320","source":"Crossref","is-referenced-by-count":22,"title":["The Dynamics of Supernumerary Tooth Development Are Differentially Regulated by Sprouty Genes"],"prefix":"10.1002","volume":"320","author":[{"given":"SVATAVA","family":"LAGRONOVA\u2010CHURAVA","sequence":"first","affiliation":[{"name":"Department of Teratology Institute of Experimental Medicine AS CR Prague Czech Republic"},{"name":"Department of Anthropology and Human Genetics Faculty of Science, Charles University Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FRANTISEK","family":"SPOUTIL","sequence":"additional","affiliation":[{"name":"Department of Teratology Institute of Experimental Medicine AS CR Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SIMONA","family":"VOJTECHOVA","sequence":"additional","affiliation":[{"name":"Department of Teratology Institute of Experimental Medicine AS CR Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HERVE","family":"LESOT","sequence":"additional","affiliation":[{"name":"INSERM UMR 1109, Team \u201cOsteoarticular and Dental Regenerative NanoMedicine\u201d, and Dental School Universit\u00e9 de Strasbourg Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MIROSLAV","family":"PETERKA","sequence":"additional","affiliation":[{"name":"Department of Teratology Institute of Experimental Medicine AS CR Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"OPHIR D.","family":"KLEIN","sequence":"additional","affiliation":[{"name":"Departments of Orofacial Sciences and Pediatrics and Program in Craniofacial and Mesenchymal Biology University of California San Francisco San Francisco, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RENATA","family":"PETERKOVA","sequence":"additional","affiliation":[{"name":"Department of Teratology Institute of Experimental Medicine AS CR Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,4,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.5962\/bhl.title.46862"},{"key":"e_1_2_6_3_1","first-page":"319","article-title":"Comparison of growth and cell\u2010proliferation kinetics during mouse molar odontogenesis in vivo and in vitro","volume":"20","author":"Ahmad N","year":"1987","journal-title":"Cell Tissue Kinet"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.054668"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910508400304"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7580.2008.01014.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.2754\/avb199867010003"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.20804"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2010.01.007"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345010800110501"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.04.021"},{"key":"e_1_2_6_12_1","volume-title":"On the origin of species. 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