{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T14:56:02Z","timestamp":1779893762192,"version":"3.53.1"},"reference-count":23,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,1,25]],"date-time":"2017-01-25T00:00:00Z","timestamp":1485302400000},"content-version":"vor","delay-in-days":55,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["81381"],"award-info":[{"award-number":["81381"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Exp Zool Pt B"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Absence of large amounts of orofacial tissues caused by cancerous resections, congenital defects, or trauma results in sequelae such as dysphagia and noticeable scars. Oral\u2010neck tissue regeneration was studied in the axolotl (regenerative amphibian) following a 2.5\u2010mm punch biopsy that simultaneously removed skin, connective tissue, muscle, and cartilage in the tongue and intermandibular region. The untreated wound was studied macroscopically and histologically at 17 different time points ranging from 0 to180 days (<jats:italic>N<\/jats:italic> = 120 axolotls). At 12 hr, the wound's surface was smoothened and within 1mm, internal lingual muscular modifications occurred; at the same distance, between days 4\u20137 lingual muscle degradation was complete. Immunofluorescence indicates complete keratinocytes migration by 48 hr. These cells with epidermal Leydig cells, appearing yellow, lead the chin's deep tissue outgrowth until its closure on the 14th day. Regeneration speeds varied and peaked in time for each tissue, (1) deep chin 84.3 \u03bcm\/hr from 24 to 96 hr, (2) superficial chin 71.1 \u03bcm\/hr from 7\u201314 days, and (3) tongue 86.0 \u03bcm\/hr between 48 hr and 7 days. Immunofluorescence to Col IV showed basement membrane reconnected between days 30\u201345 coinciding with the chin's dermal tissue's surface area recovery. New muscle appeared at 21 days and was always preceded by the formation of a collagen bed. Both chin tissues regain all surface area and practically all components while the lingual structure lacks some content but is generally similar to the original. The methodology and high\u2010resolution observations described here are the first of its kind for this animal model and could serve as a basis for future studies in oral and facial regenerative research.<\/jats:p>","DOI":"10.1002\/jez.b.22723","type":"journal-article","created":{"date-parts":[[2017,1,25]],"date-time":"2017-01-25T16:06:15Z","timestamp":1485360375000},"page":"489-502","source":"Crossref","is-referenced-by-count":13,"title":["Oral\u2010Facial Tissue Reconstruction in the Regenerative Axolotl"],"prefix":"10.1002","volume":"326","author":[{"given":"Andre M.","family":"Charbonneau","sequence":"first","affiliation":[{"name":"Craniofacial Tissue Engineering and Stem Cells Laboratory Faculty of Dentistry McGill University  Montreal Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephane","family":"Roy","sequence":"additional","affiliation":[{"name":"Department of Stomatology University de Montreal  Montreal Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon D.","family":"Tran","sequence":"additional","affiliation":[{"name":"Craniofacial Tissue Engineering and Stem Cells Laboratory Faculty of Dentistry McGill University  Montreal Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,1,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.12.040"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1401210207"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-2495-0_3"},{"key":"e_1_2_6_5_1","first-page":"807","article-title":"Re\u2010examining jaw regeneration in urodeles: what have we learnt?","volume":"40","author":"Ferretti P","year":"1996","journal-title":"Int J Dev Biol"},{"key":"e_1_2_6_6_1","first-page":"479","article-title":"Regenerative capability of upper and lower jaws in the 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