{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T00:08:38Z","timestamp":1771546118965,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,2,13]],"date-time":"2017-02-13T00:00:00Z","timestamp":1486944000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Guangdong Province Science and Technology Plans","award":["2016A050502029"],"award-info":[{"award-number":["2016A050502029"]}]},{"name":"Foundation of Ministry of Agriculture","award":["2015Z019"],"award-info":[{"award-number":["2015Z019"]}]},{"name":"Guangdong Province Marine Fishery Science technology and industry","award":["A201501A01"],"award-info":[{"award-number":["A201501A01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Exp Zool Pt B"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>vasa<\/jats:italic> gene encodes a highly conserved DEAD\u2010box RNA helicase, required for germ cell development across animal kingdom. <jats:italic>Vasa<\/jats:italic> mutations cause male infertility in mammals. It has been widely used as a biomarker for studying animal fertility or manipulating germ cells in organisms. However, in reptilians, the functions of <jats:italic>vasa<\/jats:italic> gene involved in germ cell differentiation are largely unclear; this hampers the development of biological techniques and the improvement of the productivity in these species. Here a <jats:italic>vasa<\/jats:italic> cDNA was isolated in Chinese soft\u2010shell turtle and it predicts a protein of 691 amino acid residues, which is 72%, 69%, 58%, 59%, and 54\u201356% identical to its homolog from mouse, platypus, frog, chicken, and fish, respectively, and named as <jats:italic>PsVasa<\/jats:italic>. The <jats:italic>Psvasa<\/jats:italic> mRNA was detected exclusively in the gonads of both sexes by RT\u2010PCR. Chromogenic RNA in situ hybridization revealed that the <jats:italic>Psvasa<\/jats:italic> mRNA was restricted to germ cells in the testis: The <jats:italic>psvasa<\/jats:italic> mRNA is undetectable in resting spermatogonia, appears in proliferating spermatogonia, and becomes abundant in spermatocytes and detectable in spermatozoa. Immunofluorescence staining demonstrated that the PsVasa in the testis is also restricted to the germ cells, rich in spermatocytes and elongated spermatids but hardly detectable in spermatogonia and spermatozoa. Taken together, <jats:italic>Psvasa<\/jats:italic> is potentially a reliable germ cell marker in the Chinese soft\u2010shell turtle; its RNA expression could distinguish the different spermatogenic stages of germ cells. These findings shed new insights into understanding the evolutionary conservations and divergences of <jats:italic>vasa<\/jats:italic> gene's functions in male germ cell differentiation in metazoans.<\/jats:p>","DOI":"10.1002\/jez.b.22728","type":"journal-article","created":{"date-parts":[[2017,2,13]],"date-time":"2017-02-13T07:32:42Z","timestamp":1486971162000},"page":"230-239","source":"Crossref","is-referenced-by-count":19,"title":["A Novel Dynamic Expression of vasa in Male Germ Cells during Spermatogenesis in the Chinese Soft\u2010Shell Turtle (<i>Pelidiscus sinensis<\/i>)"],"prefix":"10.1002","volume":"328","author":[{"given":"Wei","family":"Li","sequence":"first","affiliation":[{"name":"Pearl River Fisheries Research Institute Chinese Academic of Fisheries Sciences  Guangzhou People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Piaoyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Pearl River Fisheries Research Institute Chinese Academic of Fisheries Sciences  Guangzhou People's Republic of China"},{"name":"College of Fisheries and Life Science Shanghai Ocean University  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuling","family":"Wu","sequence":"additional","affiliation":[{"name":"Pearl River Fisheries Research Institute Chinese Academic of Fisheries Sciences  Guangzhou People's Republic of China"},{"name":"College of Fisheries and Life Science Shanghai Ocean University  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinping","family":"Zhu","sequence":"additional","affiliation":[{"name":"Pearl River Fisheries Research Institute Chinese Academic of Fisheries Sciences  Guangzhou People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyan","family":"Xu","sequence":"additional","affiliation":[{"name":"Pearl River Fisheries Research Institute Chinese 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