{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:33:49Z","timestamp":1775003629579,"version":"3.50.1"},"reference-count":54,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,3,30]],"date-time":"2018-03-30T00:00:00Z","timestamp":1522368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["288797\/254969"],"award-info":[{"award-number":["288797\/254969"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Exp Zool Pt B"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The <jats:italic>Sox9<\/jats:italic> gene is important for determining sex in vertebrates, as well as for maintaining testis morphology and fertility during adult life. In the same way, <jats:italic>Vasa<\/jats:italic> is an important gene for the maintenance of the germinal lineage and has been highly conserved throughout evolution, as it is expressed in germ cells of both vertebrates and invertebrates. In the particular case of crocodiles, the expression of <jats:italic>Sox9<\/jats:italic> during gonadal morphogenesis and in the testes of 3\u2010month\u2010old <jats:italic>Alligator mississippiensis<\/jats:italic> has been studied. However, it is interesting to carry out studies on other species of crocodiles in relation to their particular mechanism for sex determination influenced by temperature. In this work, we investigated the expression of the <jats:italic>Sox9<\/jats:italic>, <jats:italic>Vasa<\/jats:italic>, <jats:italic>Foxl2<\/jats:italic>, and <jats:italic>TRPV4<\/jats:italic> genes in the ovaries and testes of 5\u2010year\u2010old juvenile crocodiles from <jats:italic>Crocodylus moreletii<\/jats:italic>. As expected, <jats:italic>Sox9<\/jats:italic> expression was found in males, but surprisingly, it was also found in females. For the first time, the expression of <jats:italic>Vasa<\/jats:italic> was reported in spermatogonia, oogonia, and oocytes of 5\u2010year\u2010old crocodiles. <jats:italic>Foxl2<\/jats:italic> is important for the development and maintenance of the ovary during adult life in vertebrates; moreover, <jats:italic>Foxl2<\/jats:italic> protein and transcripts are both highly expressed in the ovaries compared to the testes. A possible upstream regulator of the <jats:italic>Sox9<\/jats:italic> gene in reptiles has not yet been discovered; as such, the expression of the TRPV4 ion channel was evaluated. The TRPV4 ion channel was expressed in the cytoplasm of Sertoli and follicular cells and was therefore proposed as a possible regulator of SOX9.<\/jats:p>","DOI":"10.1002\/jez.b.22799","type":"journal-article","created":{"date-parts":[[2018,3,30]],"date-time":"2018-03-30T23:07:59Z","timestamp":1522451279000},"page":"148-164","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Expression of the <i>Sox9<\/i>, <i>Foxl2<\/i>, <i>Vasa<\/i>, and <i>TRPV4<\/i> genes in the ovaries and testes of the Morelet's crocodile, <i>Crocodylus moreletii<\/i>"],"prefix":"10.1002","volume":"330","author":[{"given":"Adriana","family":"Mart\u00ednez\u2010Ju\u00e1rez","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda Celular y Fisiolog\u00eda Instituto de Investigaciones Biom\u00e9dicas UNAM  Mexico Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco A.","family":"L\u00f3pez\u2010Luna","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n Acad\u00e9mica de Ciencias Biol\u00f3gicas Universidad Ju\u00e1rez Aut\u00f3noma de Tabasco Villahermosa Tabasco  Mexico Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tania J.","family":"Porras\u2010G\u00f3mez","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Celular y Fisiolog\u00eda Instituto de Investigaciones Biom\u00e9dicas UNAM  Mexico Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3508-799X","authenticated-orcid":false,"given":"Norma","family":"Moreno\u2010Mendoza","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Celular y Fisiolog\u00eda Instituto de Investigaciones Biom\u00e9dicas UNAM  Mexico Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000447611"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(199910)216:2<153::AID-DVDY6>3.0.CO;2-1"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"261","DOI":"10.22201\/ib.20078706e.2011.1.444","article-title":"Reproducci\u00f3n en cautiverio de Crocodylus moreletii en Tabasco, M\u00e9xico","volume":"82","author":"Casas\u2010Andreu G.","year":"2011","journal-title":"Revista Mexicana De Biodiversidad"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.0129"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.39.12.916"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402700112"},{"key":"e_1_2_6_8_1","first-page":"211","article-title":"Scientific results of an inquiry into the ecology and economic status of the Nile crocodile (Crocodylus niloticus) in Uganda and Northern Rhodesia","volume":"29","author":"Cott H. 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