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In model organisms, regeneration usually occurs as the induction of proliferation in differentiated cells. In rare circumstances, regeneration can be the product of na\u00efve progenitor cells proliferating and differentiating de novo\n<jats:italic>.<\/jats:italic> In any instance, the immune system is an important factor in the induction of the regenerative response. Although the wound response is well\u2010characterized, little is known about the physiological pathways utilized by cuttlefish to reconstruct a lost arm. In this study, the regenerating arms of juvenile cuttlefish, with or without exposure at the time of injury to sterile bacterial lipopolysaccharide extract to provoke an antipathogenic immune response, were assessed for the transcription of early tissue lineage developmental genes, as well as histological and protein turnover analyses of the resulting regenerative process. The transient upregulation of tissue\u2010specific developmental genes and histological characterization indicated that coleoid arm regeneration is a stepwise process with staged specification of tissues formed de novo, with immune activation potentially affecting the timing but not the result of this process. Together, the data suggest that rather than inducing proliferation of mature cells, developmental pathways are reinstated, and that a pool of na\u00efve progenitors at the blastema site forms the basis for this regeneration.<\/jats:p>","DOI":"10.1002\/jez.b.22849","type":"journal-article","created":{"date-parts":[[2019,3,20]],"date-time":"2019-03-20T03:33:38Z","timestamp":1553052818000},"page":"113-120","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Reversion to developmental pathways underlies rapid arm regeneration in juvenile European cuttlefish, <i>Sepia officinalis<\/i> (Linnaeus 1758)"],"prefix":"10.1002","volume":"332","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8214-3395","authenticated-orcid":false,"given":"Neal I.","family":"Callaghan","sequence":"first","affiliation":[{"name":"Institute of Biomaterials and Biomedical Engineering, Faculty of Applied Science and Engineering, University of Toronto 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