{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T07:33:38Z","timestamp":1777793618126,"version":"3.51.4"},"reference-count":42,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2021,3,10]],"date-time":"2021-03-10T00:00:00Z","timestamp":1615334400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Exp Zool Pt B"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The notochord functions primarily as a supporting tissue to maintain the anteroposterior axis of primitive chordates, a function that is replaced entirely by the vertebral column in many vertebrates. The notochord still appears during vertebrate embryogenesis and plays a crucial role in the developmental pattern formation of surrounding structures, such as the somites and neural tube, providing the basis for the vertebrate body plan. The indispensable role of the notochord has often been referred to as the developmental burden and used to explain the evolutionary conservation of notochord; however, the existence of this burden has not been successfully exemplified so far. Since the adaptive value of target tissues appears to result in the evolutionary conservation of upstream structures through the developmental burden, we performed comparative gene expression profiling of the notochord, somites, and neural tube during the mid\u2010embryonic stages in turtles and chicken to measure their evolutionary conservation. When compared with the somites and neural tube, overall gene expression profiles in the notochord showed significantly lower or merely comparable levels of conservation. However, genes involved in inductive signalings, such as the sonic hedgehog (\n                    <jats:italic>Shh<\/jats:italic>\n                    ) cascade and the formation of functional primary cilia, showed relatively higher levels of conservation in all the three structures analyzed. Collectively, these results suggest that\n                    <jats:italic>shh<\/jats:italic>\n                    signals are critical as the inductive source and receiving structures, possibly constituting the inter\u2010dependencies of developmental burden.\n                  <\/jats:p>","DOI":"10.1002\/jez.b.23032","type":"journal-article","created":{"date-parts":[[2021,3,10]],"date-time":"2021-03-10T09:42:07Z","timestamp":1615369327000},"page":"129-136","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Measuring potential effects of the developmental burden associated with the vertebrate notochord"],"prefix":"10.1002","volume":"338","author":[{"given":"Satoko","family":"Fujimoto","sequence":"first","affiliation":[{"name":"RIKEN, Center for Biosystems Dynamics Research  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaori","family":"Yamanaka","sequence":"additional","affiliation":[{"name":"RIKEN, Center for Biosystems Dynamics Research  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiharu","family":"Tanegashima","sequence":"additional","affiliation":[{"name":"RIKEN, Center for Biosystems Dynamics Research  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osamu","family":"Nishimura","sequence":"additional","affiliation":[{"name":"RIKEN, Center for Biosystems Dynamics Research  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigehiro","family":"Kuraku","sequence":"additional","affiliation":[{"name":"RIKEN, Center for Biosystems Dynamics Research  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9717-7221","authenticated-orcid":false,"given":"Shigeru","family":"Kuratani","sequence":"additional","affiliation":[{"name":"RIKEN, Center for Biosystems Dynamics Research  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6720-381X","authenticated-orcid":false,"given":"Naoki","family":"Irie","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences The University of Tokyo  Tokyo Japan"},{"name":"Universal Biology Institute The University of Tokyo  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,3,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2013.09.004"},{"key":"e_1_2_9_3_1","volume-title":"Conceptual change in biology: Scientific and philosophical perspectives on evolution and development","author":"Alan C. 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