{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T19:57:48Z","timestamp":1785182268379,"version":"3.55.0"},"reference-count":65,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T00:00:00Z","timestamp":1691452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Exp Zool Pt B"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Kryptolebias marmoratus<\/jats:italic>\n                    (Kmar), a teleost fish of the order Cyprinodontiformes, has a suite of unique phenotypes and behaviors not observed in other fishes. Many of these phenotypes are discrete and highly plastic\u2014varying over time within an individual, and in some cases reversible. Kmar and its interfertile sister species,\n                    <jats:italic>K. hermaphroditus<\/jats:italic>\n                    , are the only known self\u2010fertile vertebrates. This unusual sexual mode has the potential to provide unique insights into the regulation of vertebrate sexual development, and also lends itself to genetics. Kmar is easily adapted to the lab and requires little maintenance. However, its internal fertilization and small clutch size limits its experimental use. To support Kmar as a genetic model, we compared alternative husbandry techniques to maximize recovery of early cleavage\u2010stage embryos. We find that frequent egg collection enhances yield, and that protease treatment promotes the greatest hatching success. We completed a forward mutagenesis screen and recovered several mutant lines that serve as important tools for genetics in this model. Several will serve as useful viable recessive markers for marking crosses. Importantly, the mutant\n                    <jats:italic>kissylips<\/jats:italic>\n                    lays embryos at twice the rate of wild\u2010type. Combining frequent egg collection with the\n                    <jats:italic>kissylips<\/jats:italic>\n                    mutant background allows for a substantial enhancement of early embryo yield. These improvements were sufficient to allow experimental analysis of early development and the successful mono\u2010 and bi\u2010allelic targeted knockout of an endogenous\n                    <jats:italic>tyrosinase<\/jats:italic>\n                    gene with CRISPR\/Cas9 nucleases. Collectively, these tools will facilitate modern developmental genetics in this fascinating fish, leading to future insights into the regulation of plasticity.\n                  <\/jats:p>","DOI":"10.1002\/jez.b.23216","type":"journal-article","created":{"date-parts":[[2023,8,9]],"date-time":"2023-08-09T01:23:36Z","timestamp":1691544216000},"page":"164-177","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Genetic tools for the study of the mangrove killifish,\n                    <i>Kryptolebias marmoratus<\/i>\n                    , an emerging vertebrate model for phenotypic plasticity"],"prefix":"10.1002","volume":"342","author":[{"given":"Cheng\u2010Yu","family":"Li","sequence":"first","affiliation":[{"name":"Department of Biology University of Maryland College Park Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"Boldt","sequence":"additional","affiliation":[{"name":"Department of Genetics Harvard Medical School Boston Massachusetts USA"},{"name":"Department of Orthopaedic Surgery Boston Children's Hospital Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Parent","sequence":"additional","affiliation":[{"name":"Department of Biology University of Maryland College Park Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jax","family":"Ficklin","sequence":"additional","affiliation":[{"name":"Department of Biology University of Maryland College Park Maryland USA"},{"name":"College of Computer, Mathematical, and Natural Sciences, Biological Sciences Graduate Program University of Maryland College Park Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Althea","family":"James","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Boston Children's Hospital Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Troy J.","family":"Anlage","sequence":"additional","affiliation":[{"name":"Department of Biology University of Maryland College Park Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lena M.","family":"Boyer","sequence":"additional","affiliation":[{"name":"Department of Biology University of Maryland College Park Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brianna R.","family":"Pierce","sequence":"additional","affiliation":[{"name":"Department of Biology University of Maryland College Park Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kellee R.","family":"Siegfried","sequence":"additional","affiliation":[{"name":"Department of Biology University of Massachusetts Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew P.","family":"Harris","sequence":"additional","affiliation":[{"name":"Department of Genetics Harvard Medical School Boston Massachusetts USA"},{"name":"Department of Orthopaedic Surgery Boston Children's Hospital Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6102-3498","authenticated-orcid":false,"given":"Eric S.","family":"Haag","sequence":"additional","affiliation":[{"name":"Department of Biology University of Maryland College Park Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09164"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2022.107617"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2019.08.002"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.950170513"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku936"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-05612-8"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2021.08.011"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.123.1.37"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/icb\/ics118"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0706.2012.20556.x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1701508"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.123.1.1"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.2307\/1539887"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.134.3492.1749"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1086\/physzool.36.4.30152746"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1086\/282547"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.2307\/1440553"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00360-017-1123-4"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2019.10.004"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12111"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1006\/anbe.1998.1049"},{"issue":"1","key":"e_1_2_9_24_1","first-page":"1","article-title":"Notes 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