{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T06:45:51Z","timestamp":1786430751370,"version":"build-2736575974"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T00:00:00Z","timestamp":1700438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["Hatch project LAB94420"],"award-info":[{"award-number":["Hatch project LAB94420"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Exp Zool Pt B"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Sperm cryopreservation is a critical tool for safeguarding and managing valuable genetic resources. Protocols for cryopreservation of\n                    <jats:italic>Xenopus laevis<\/jats:italic>\n                    sperm were available but lacking sperm quality evaluation and scalability and the outcomes were inconsistent. The goal of this study was to begin developing a center\u2010level cryopreservation pathway for this species by integrating French straws as containers that would facilitate germplasm repository development. The objectives were to analyze the effect of: (1) three sperm concentrations (33, 50, and 100\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    sperm\/mL) on post\u2010thaw fertilization, (2) three final concentrations (2.5%, 5%, and 10%) of dimethyl sulfoxide, methanol, and dimethylformamide (DMFA) on sperm membrane integrity of fresh and frozen samples, (3) two concentrations (5% and 10%) of DMFA with and without 5% sucrose at four cooling rates (5, 10, 20, and 40\u00b0C\/min) on sperm membrane integrity and motility, and (4) egg exposure to different concentrations of DMFA on fertilization. Few differences in sperm viability were found among fresh samples incubated in cryoprotectants, but thawed samples frozen in methanol or DMFA presented higher membrane integrity. Samples frozen in 10%\u00a0DMFA at 20\u00b0C\/min showed higher membrane integrity (60\u2009\u00b1\u20097%) than other DMFA concentrations and cooling rates, and the same total motility (30\u2009\u00b1\u20097%) as at 10\u00b0C\/min. Higher DMFA concentrations (10%\u201313%) were detrimental for embryo development compared to lower concentrations (&lt;6%). This study provided a reliable protocol for sperm cryopreservation in\n                    <jats:italic>Xenopus laevis<\/jats:italic>\n                    to yield an application pathway with potential for high throughput that can be used as a roadmap for work with other species.\n                  <\/jats:p>","DOI":"10.1002\/jez.b.23228","type":"journal-article","created":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T17:09:28Z","timestamp":1700586568000},"page":"291-300","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Transitioning from a research protocol to a scalable applied pathway for\n                    <i>Xenopus laevis<\/i>\n                    sperm cryopreservation at a national stock center: The effect of cryoprotectants"],"prefix":"10.1002","volume":"342","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4355-0155","authenticated-orcid":false,"given":"Luc\u00eda","family":"Arregui","sequence":"first","affiliation":[{"name":"School of Renewable Natural Resources,\u00a0Aquatic Germplasm and Genetic Resources Center Louisiana State University Agricultural Center Baton Rouge Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack C.","family":"Koch","sequence":"additional","affiliation":[{"name":"School of Renewable Natural Resources,\u00a0Aquatic Germplasm and Genetic Resources Center Louisiana State University Agricultural Center Baton Rouge Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terrence R.","family":"Tiersch","sequence":"additional","affiliation":[{"name":"School of Renewable Natural Resources,\u00a0Aquatic Germplasm and Genetic Resources Center Louisiana State University Agricultural Center Baton Rouge Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.10.013"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2020.735677"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/zeb.2022.0006"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.2983\/035.041.0206"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/cryo.1998.2129"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20005"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/csp2.525"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2006.05.011"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2006.11.007"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2013.06.013"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD02079"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2013.02.018"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2011.08.003"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2019.05.007"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2017.03.010"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2013.07.001"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2003.09.019"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani12192537"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.4081\/ijas.2009.s2.760"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2021.106871"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.07.013"},{"key":"e_1_2_8_23_1","doi-asserted-by":"crossref","unstructured":"Mazur P. Leibo S. P. Farrant J. Chu E. H. Y. Hanna M. G. &Smith L. H.(1970).Interactions of cooling rate warming rate and protective additive on the survival of frozen mammalian cells. In Ciba Foundation Symposium\u2014The Frozen Cell\u00a0(pp.69\u201388).","DOI":"10.1002\/9780470719732.ch5"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD16190"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot107029"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2017.01.007"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.20092"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature19840"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.08.008"},{"key":"e_1_2_8_30_1","first-page":"646","volume-title":"Cryopreservation in aquatic species","author":"Tiersch T. 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